Full text of "USPTO Patents Application 10551504"

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PCT/JP 2001/018506 



H * I # ff )j 14.12.2004 



JAPAN PATENT OFFICE 



wiTnTajl Office"' 1, <hl ' tb ° a " DeXed is a true c °Py ° f following application as filed 



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Date of Application: 2004^ 3^120 
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Application Number: ^® 2004-071763 

[ST. io/C] : CJP2004-071763] 

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(15) mm-% : 4 5, 4 6, 4 7 

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(20) IB?nj#-^ : 2 3 1 , 233, 235 

(21) mm-% 1266, 268, 270 

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(2) BB?!I#-^ 1265, 267, 269, 271 

(3) @S^1J#^-: 279, 281, 283, 285 

(1) @5^IJ## 1239, 241, 243, 245 

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* (Matthias Balmier et a BLOM f *? f o^f "* tt ^«**^^ 

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fi*mfctt;#li, wholes tit«LT 

*w* (vl) ^ v »4£t^if^ wtt m x*/*tmmT* 

le * v Diabod , sc(Fv)2 ssi.rss 

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; CDR) #ffi S fttfi U yH-YL^^*1^^i2S^SL arity detenninin S region 

iiu **** *° ' ^ **-«ffiJ: *> ta«ttttfiv»^ aac*. 

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at>4> CHudson et aU J Immunol. Methods 1999 ; 231 : 177-189) 0 sc(Fv)2t± ^Mtirfc 

««^J:v^ CVH] m ) 

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[VL] y>#- [VH] y>#- [vh] y>#- [vl] 
[vh] y>*- [vl] y>#- [vl] y>*- [vh] 
[vh] y>#- [vh] y>#- [vl] y>#- [vl] 
[vl] y>#- [vl] y^a- [vh] y>*- [vh] 
[vl] [vh] y>#- [vl] y^a- [yhI 

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f 199U2r;-5«f?^r^I P-tein Engineering, 9(3), 299-305 

S^fJw^*™*** 5 ^ 1-lOOT^m. #4L<tt3~50T5y» 

j ^»*U<»i5-307 5yBfc, Wfcff*L<ttl2~187^Bfc 1575yi 

[0 0 2 6] 

Ser 

Gly • Ser 

Gly • Gly - Ser 

Ser • Gly • Gly 

Gly • Gly • Gly • Ser 

Ser • Gly • Gly • Gly 

Gly • Gly • Gly • Gly • Ser 

Ser • Gly • Gly • Gly • Gly 

Gly • Gly • Gly • Gly • Gly • Ser 

Ser • Gly • Gly • Gly • Gly • Gly 

Gly • Gly • Gly • Gly • Gly • Gly • Ser 

Ser • Gly • Gly • Gly • Gly • Gly ■ Gly 

(Gly • Gly • Gly • Gly • Ser)n 

(Ser • Gly • Gly • Gly • Gly)n 

_i?yf* M l?* v ^Wfc»*Lv>sc(FV)2©««fcLTtt, &TOsc(Fv)2£ 

2pr*7 -O <_ C. 7)^X 5 J„ 

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#12 0 0 4-0 7 1 7 6 3 ^~>?- 9/ 

* ™s7-toX?mVx' m ^'" J5 '*-<»T^W [VH] ^ 

[0 0 2 8] 

«^?f^^^ b (BS3) * ***** u^>w^^ 

(*****s,>.f 3 M (* ; i.*-EGS) y^-vw^ 
(DST) , VXA,*X**,>4 U^-TO //^U 

+ (BS0C0ES) . tr* [2- 

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[0 0 2 9] 

(SSj'l^Si 29 P^ 6 : B : tt6r ' ^ ^ H °- itZ ' Method sU-Ji. 

(1989) 178 476-496 Pluckthun, A. and Skerra, A., Methods Enzymol. (1989) 178 

. u ! a L f 0yi, 1 E " / Met ?° ds EnzymoL (1986) 12l > 652 - 6 63 : Rousseaux, J. et a 
1 Methods Enzymol. (1986) 121, 663-669 ; Bird, R. E. and Walker B W Trends 
Biotechnol. (1991) 9, 132-137#HS) 0 ' W ' ' lrends 

[0 0 3 0] 

V!?? ?*? ****** **• ^6 TTM 

[0 0 3 1 ] 

[0 0 3 2] . - - 



t MbSL#(± J« (reshaped) dfltt, fc h jglftoiftaj&tt 

**»tt*fe«* (CDR; complementarity determining region) * 

*LTV*4 (RWW^tt K «n #W 125023-^iU W0 96702576^^ 0 



[0 0 3 3] 

«*^^+^M^ C ? fct ha#«7WA7^«* (framework region ; F 
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#® 2004-071763 ^5- • 10/ 

[0?!ff (Sat °' K - eUl " Ca " CerReS - (1993> »• 851-856) ° 

tS^C^^^'T?^^';'*- K, -«#®***«tUliSft. MUM 

™* ( "** ro ****** 

**™?«*i"^4V* ^ ***** 

[0 0 3 8] 

^* raw -^rV l^i-fT** : 2 2 9 (k Hfc««ra : hVB22B P 

o ^ w, *Ir^ -2 5 6 (t MbSiJ^J : hVB22B g-e YH) VM±iE5i|#-& • ? « 

s cffi«or§ >>*K9y**-r«e«TO*tt a^kew^t . 2 3 

8 fcffifcor ^ ^*K3?fl ««rTR«* J**W. ACMHfl|#* . 2 5 

[0 0 4 0] 

[0 0 4 1] 

hVB22B p-z VH^mSMe^S-ia^lJ#^- : 2 2 8 , hVB22B g-e W<Dt*&Wm frraM- • 

i 5 2 s f ^T^57^»* : 2 6 ^ hvB22B « ^Kra«£i 

^.2 3 7, hVB22B g-e VL& <£ O f hVB22B e VLO«E3F0*E?UW : 2 5 7 KE*?J 
[0 0 4 2] 

: hVB22B g- e VH) , ^, 1KJM : 2 P g 2 (l. h S^-j hVB22B . v^- 

wemoT^ ^^ie^iju^v^t Membra . hVB22B e vh) k_ 

T^y»-^: 3 1-3 5^CDR1, 
T 5 y : 5 0-66 tf*CDR2, 

7 ^»: 9 9-10 7*»CDR3, 



tbmE#2 005-3004069 



#12 0 0 4-0 7 1 7 6 3 • u 

7 ^ y : i~30 *«eri, 

: 3 6-4 9ri*FR2, 
7^®ff : 67-9 8j&*FR3. 
T5ym#-t: 10 8-11 8ifiFR4izifcM-tZ 0 
[0 0 4 3] 

X, mmm^ 12 3 8 (t Mfrgfll^iJ : hVB22B p-z VL) SfcttiRWIS-S- • 2 «? 8 C 

xrissf^r 2 3 B 9 6 ^* feahvB22B e w ( 

7 ^ : 5 5-61 #CDR2, 

7 5 J :94~102 ri*CDR3, 

7^itf : i - 2 3 ami, 

T<S : 4 0 - 5 4 tf*FR2, 

T 5 y : 6 2 - 9 3 #FR3, 

7?y itf : 10 3-11 2#FR4tffia-r&o 
[0 0 4 4 ] 

a***« 0 * v * T% hVB22B P-z ™^ WCDR ^»^w^twii^T^ 

hVB22B p-z VH : FRl/Ba^J#-§- '230 
hVB22B p-z VH : CDR1/IS^IJ#^- : 2 3 1 
hVB22B p-z VH : FR2/ / @e^!]#-§- : 232 
hVB22B p-z VH : CDR2/gB^J#-^ : 2 3 3 
hVB22B p-z VH : FR3/MB?iJ#^- : 2 3 4 
hVB22B p-z VH : CDR3/I£yy#-S§- ; 235 
hVB22B p-z VH : FR4/@E^iJ#-t : 236 
[0 0 4 5] 

fl***5bf V ' T% hVB22B P " Z ^^'^CDR^i^tTOft^ii^ 
hVB22B p-z VL : FRl/|By!l#-f- 12 3 9 
hVB22B p-z VL : CDRl/ffi^J*-^- : 2 4 0 
hVB22B p-z VL : FR2/I2^J#-^- : 2 4 1 
hVB22B p-z VL : CDR2/Mg^J#-^- : 2 4 2 
hVB22B p-z VL : FR3/g^J#-5§- ; 243 
hVB22B p-z VL : CDR3/@e^J#-^- : 2 4 4 
hVB22B p-z VL I FR4/@e^J#-*§- '245 
[0 0 4 6] 

hVB22B g-e VH : FRl/MB^iJ#^- : 265 
hVB22B g-e VH : CDRl/BE?!l#-!§- : 2 6 6 
hVB22B g-e VH : FR2/@e^!J#-^ : 2 6 7 
hVB22B g-e VH : CDR2/1E?!J#-S§- : 2 6 8 
hVB22B g-e VH : FR3/@E?iJ#-§- : 269 
hVB22B g-e VH : CDR3/@B^!l#-^ : 270 
hVB22B g-e VH : FR4/gB2?lJ#^- '271 
[0 0 4 7] 

hVB22B g-e VL : ERl/K3W-§- : 2 7 2 
hVB22B g-e VL : CDRl/M&m^ 12 7 3 
hVB22B g-e VL : FR2/HB2?!l#-^- : 2 7 4 



£BIiE#2 005-3004069 



2004-071763 



^-v: 12/ 



hVB22B g-e VL I CDR2Xie5 r iJ#-^- : 275 
hVB22B g-e VL : FR3Xmm^ I 2 7 6 
hVB22B g-e VL I CDR3/@g^J#-^- : 2 7 7 
hVB22B g-e VL : FR4/IB?iJ#^- "278 
[0 0 4 8] 

*^3KJ3V>T, hVB22B e VHEgl fc*»t * CDR£ J: OTK ©#i&*±£lT«>» 

hVB22B e VH : FRl/B2£UlHg- : 279 
hVB22B e VH : CDRl/Se^iJig^- : 2 8 0 
hVB22B e VH : FR2/S2^iJ#-§- : 2 8 1 
hVB22B e VH : CDR2/Wij#-§- : 282 
hVB22B e VH : FR3/iB?iJ## : 2 8 3 
hVB22B e VH : CDR3/@e^iJ#-^ 12 8 4 
hVB22B e VH : FR4/gB?iJ#-§- : 2 8 5 
10 0 4 9] 

^mz^X, hVB22B e VUE8I S »t * CDR* J: OTRfcEyi]** *>*ttSlija T ©a 

hVB22B e VL : FRl/ffi^J*^- 12 7 2 
hVB22B e VL : CDR1/@B?|J## : 2 7 3 
hVB22B e VL : FR2/ie^ij#^- ; 274 
hVB22B e VL : CDR2/@e^J#-5§- : 275 
hVB22B e VL : FR3/WlJ#-§- 12 7 6 
hVB22B e VL : CDR3/@5^J#-^- : 2 7 7 
hVB22B e VL : FR4/@E?iJ#^- : 278 
[0 0 5 0] 

hVB22B p-z VHBE?lJi3.fctfhVB22B p-z VLBE^lJ ^ ^ it & CDR* J: 0*M WJfc * , H 
1 8 fr^^c L7t 0 

[0 0 5 1 ] 

^k B Btt©T<y»E?!l*»e,**, FR1, 2. 3, 4 WR***** H h >ffc 

(1) @2^IJ#^: 230, 232, 234, 236 

(2) @B^iJ#^-: 265, 267, 269, 271 

(3) mm^: 2 7 9, 2 8 1 , 2 8 3, 2 8 5, 

SI2iL^i ( H£ fBmoT5y ^ @ ^ ,J ^' b ^^^ fri, 2, s, 4*mi-zmm*jmM® 

(1) S£^J#-^: 239, 241, 243, 245 

(2) mm-%: 272, 274, 276, 278 

(1) BE3W§-: 2 3 1 , 2 3 3 , 2 3 5 

(2) iB^J## : 2 6 6, 268, 270 

(3) @B^J#^-: 2 8 0, 2 8 2, 2 8 4, Xl± 

(DlSSWf- 1240, 242, 244 
(2)Efl|#*: 2 7 3, 2 7 5, 2 7 7, 

[0052] w 

tBfE# 2005-3004069 



#02 0 0 4 -0 7 1 7 6 3 i„ 
<»Kf**: 23C K 2 3 2, 2 3 4- 2 3 6l:Eltersy 8ra MM FR1 2 

*WU 2. 3**+*a^;|« 2 °- 2 4 2 ~ 2 **I-«S«»7^HS(M 4 

scdri, 2, Bimtzmmvmin 7 ^•^^areai**,* 

l 3 K?« 2 Vi4 8 ^* 2 . 8 2 4 7?T 7 7 T 7 E 7 ^ 7 CDR1 - 2 - 3 ** + 

[0 0 5 6] 

B Amm*4 la T^ ^" 1985 ; 41 : 727-734, Ga^ an k# NM _005373) , *^ 1fM 

Hitt#2 005-3004069 



#M 2004-071763 



^-v: 14/ 



[0 0 5 9] 

ff??^^ ^^^^Mpl^<7)^^tt^T(D=10- 6 MmTOSL#-C*^^^(± 

T **f tt^iSttOffHiffiKtt, ELISA (BXffi#ft&K**£& 

) , EIA (i£«&«5£&) , ria (Jfclt^ftaj^fe) *^^tt^«^v^;t^ 



[0 0 6 0] 
[0 0 6 1 ] 

LT, J^TC0(I)~(XII)^v,i-^^fB« 

[0 0 6 2] 

(I) 

4, 5 (VA7 : HilCDRl, 2. 3) 
7 . 8 (VA130 I HfflCDRl, 2, 3) 



(2) l£?y#-§- : 

(3) mm^ : 

(4) mm-% : 

(5) is^j#^- : 

(6) BB2»-5g- : 

(7) mm^- : 

(8) @E?W§- : 

(9) iB?iJ## : 

(10) BE?!I#-^ 

(12) SE^|J#^- 

(13) E3?!I#-^ 

(14) ifi^ij#-i- 

(15) BE3?1J#-§- 

(16) @2^J#^- 

(17) HB5W§- 

(21) @e?!)#-5§- 

(22) @E?!l#-5§- 
[0 0 6 3] 

(II) 



3 
6 
9 
1 
1 
1 
2 
2 
2 

3 
3 
3 
3 
4 
4 
4 
5 
5 
5 
2 
2 
2 



2, 
5, 
8, 
1 * 
4, 
7, 
0 
3 
6 
9 
2 
5 
8 
1 
4 
7 
3 
6 



0 
1 
1 
1 

2 
2 
2 

3 
3 
3 
4 
4 
4 
4 
5 
5 
5 



1 
6 
8 0 



3, 
6, 
9, 
2, 
5, 
8, 
1 
4 
7 
0 
3 
6 
9 
2 
5 
8 
2 
2 
2 



1 
1 
1 

2 
2 
2 
2 



(VA259 : HStCDRU 2, 3) 



3 
3 
3 
4 
4 
4 
5 
5 
5 
5 
3 
8 
2 



4 
7 
0 
3 
6 
9 

2 
5 
8 
1 
4 
7 
0 
3 
6 
9 



(VB17B : HfKCDRK 2, 3) 
(VB12B : HglCDRK 2, 3) 
(VB140 I HfitCDRU 2, 3) 
HiJtCDRl, 2, 3) 
IHIKDRU 2, 3) 
HIICDR1, 2, 3) 
.•HfiftCDRl, 2, 3) 

:hsicdri, 2, 3) 
IHStCDRU 2, 3) 
HfflCDRl, 2, 3) 
: HUCDRl, 2, 3) 
HfflCDRU 2> 3) 
H&CDR1, 2, 3) 
: HilCDRl, 2, 3) 
rffiWCDRU 2, 3) 
:H8ICDR1, 2, 3) 
(hVB22B p-z : H&CDR1, 2, 3) 
(hVB22B g-e : H&CDR1, 2. 3) 
(hVB22B e : H®CDR1, 2, 3) 



(VB33 
(VB45B 
(VB8B : 
(VB115 
(VB14B 
(VB22B 
(YB16 : 
(VB157 
(VB4B : 
(VB51 : 
(AB317 
(AB324 
(TA136 
2 3 5 
2 7 
2 8 



0 
4 



J±J|E#2 005-3004069 



#12004-07 1 763 



(3) @2?iJ#^- 

(4) K?!l#-f- 

(5) SB?iJ#^- 

(6) lfi?iJ#-f- 

(13) BE?0#-§- 

(14) @e^ij#-t 

(15) SH^IJ#^- 
(16) 

(17) §B^IJ#^- 

(18) IE3W§- 

(19) IS^J## 

(20) ie^iJ#^- 

(21) BB^J#-^ 
[0 0 6 4 J 



6 0 
6 3 
6 6 • 

6 9 . 

7 2 , 
7 5, 

7 8, 

8 1 , 
8 4, 

8 7 

9 0 
9 3 
9 
9 
1 
1 
1 
1 
1 
2 
2 



6 1 
6 4 



6 
7 
7 
7 
7 
8 
8 



7, 
0, 
3, 
6, 
9, 
2, 
5 , 

8 8 

9 1 



6, 
9, 
0 2 
0 5 

0 8 

1 1 



4, 
0 0 



6 2 
6 5 

6 8 

7 1 
7 4 

7 7 

8 0 
8 3 
8 6 

8 9 
9 
9 
9 



1 
4 
7 



1 
1 
1 
1 
1 
2 
2 



3 
6 
9 
2 

5 • 
2 . 
5. 



(VA7 : LilCDRK 2, 3) 
(VA130 : LgiCDRl, 2, 3) 
(VA259 : LtJtCDRl, 2, 3) 
(VB17B : LilCDRl, 2, 3) 
(VB12B : LIICDR1, 2, 3) 
(VB140 : UICDR1. 2, 3) 
(VB33 : LiJtCDRl, 2, 3) 
(VB45B : LilCDRl, 2, 3) 
(VB8B : LIKDR1, 2, 3) 
(VB115 : UKDR1, 2, 3) 
(VB14B I UJtCDRL 2. 3) 
(VB22B : UJtCDRK 2, 3) 
(VB16 : LilCDRl, 2, 3) 
0 1 (VB157 : LtlCDRl. 2. 3) 



0 4 
0 7 



0 
3 
6 
4 
7 



(VB4B : LSKDRl, 2, 3) 
(VB51 : UICDR1, 2, 3) 
(AB317 I UjtCDRl. 2, 3) 
(AB324 : UJtCDRl, 2, 3) 
(TA136 : UJtCDRl. 2, 3) 
(hVB22B p-z : LilCDRl, 2, 3) 
(hVB22B g-e% 7tfihVB22B e : UjtCDRl, 2, 3) 



(III) 

&T<D (1) ~ (22) <D^?flfr<Dmm^Z%im(DT 



(1) E?!l#-§- 

(2) iB^J#-^- 

(3) @S^!l#-t ; 

(4) se?ij#-s§- : 

(5) @S^iJ#^- ; 

(6) mm^- : 

(7) @e^ij#^- : 

(8) m&m-% : 

(12) ie^ij#^- 

(13) @E5 r iJ#-§- 

(16) @e3W§- 

(17) ie^ij#^- 

(18) @S?i|#-5§- 

(19) @EJ11#^- 

(20) ie^ij#^- 

(21) @E^lJ#-i§- 

(22) ie^ij#-t 

[0 0 6 5] 

(IV) 

&TO(l)~ 



2 4 
2 6 

2 8 

3 0 
3 2 
3 4 
3 6 

3 8 

4 0 
1 4 

4 
1 
4 
4 



1 
1 
1 
1 
1 
1 
1 
1 
1 
2 
2 
2 



6 2 



(VA7 : VH) 
(VA130 : VH) 
(VA259 : VH) 
(VB17B : VH) 
(VB12B : VH) 
(VB140 : VH) 
(VB33 : VH) 
(VB45B : VH) 
(VB8B : VH) 
(VB115 : VH) 
(VB14B : VH) 
(VB22B : VH) 
(VB16 ! VH) 
(VB157 : VH) 
(VB4B : VH) 
(VB51 : VH) 
(AB317 : VH) 
(AB324 I VH) 
(TA136 : VH) 
(hVB22B p-z 
(hVB22B g-e 
(hVB22B e 



s mmm & h 4 & vh ^ # tp^;# c 



VH) 
VH) 
VH) 



y mnm h & £ vl *• -g- & #l#: 0 

ffifE#2 005-3004069 



#H 2004-071763 



16/ 



(Die^l*-^ : 125 (VA7 : vl) 

(2) I^J#^-: 1 2 7 (VA130: VL) 

(3) i2^iJ#^: 1 2 9 (VA259:VL) 

(4) ie^j#-^-: i 3 i (vbi7B:vl) 

(5) IS^IJ#-^ : 1 3 3 (VB12B : VL) 

(6) mms§- : 1 3 5 (VB140 ' VL) 

(7) mm-%: 1 3 7 (VB33: VL) 

(8) mm-% : 1 3 9 (VB45B • VL) 

(9) m$m^ : 1 4 1 (VB8B VL) 

(10) @E^iJ#-^: 1 4 3 (VB115.-VL) 

(11) B2?tl#-*J- : 1 4 5 (VB14B ■ VL) 

(12) HE^J#-§- : 120 (VB22B • VL) 

(13) @e^iJ#-^ : 1 4 7 (VB16 : VL) 
(U)mm^ : 1 4 9 (VB157 ' VL) 

(15) @fi^iJ#-t : 1 5 1 (VB4B I VL) 

(16) @£^J#-t ; 1 5 3 (VB51 :vl) 

(17) SS^iJ#-S§- : l 5 7 (AB317 ' VL) 

(18) @2^J#-f- : 1 6 1 (AB324 • VL) 

(19) Efl|##: 1 6 3 (TA136:VL) 

(20) I£^j## ; 2 3 8 (hVB22B p-z : VL) 

(21) I2^ij#-t : 2 5 8 (hVB22B g- e : VL£ WthVB22B e : VL) 
[0 0 6 6] 

(V) 

mmuZ'3) 5 (VA? : 2 - 3) ' W,W:6 »- 6 1, 6 2 (VA7: 

3o3J;2 6 ;3) 7, 8 <VA13 ° : mCDRU * 3> - E »* : «. 6 4 , 6 5 (VA! 
( S*ic4 2° 3) 1 1 H3WCDR1, 2. 3) , ; 6 6 , 6 7. 6 8 

ra^imiAV* <VBm:HfflCDR1 - 2 > 3).B««:6.. 70. 7 
4 TS^i. ' 2 6 3, 1 7 (VB12B : 2 - 3) ' K ^ =72,73,7 

fmuoVmml Yh 2 ° (VB140: ~~ 2 ' 3) ' 7 6, 7 6, 7 

^Sckl^,', 2 - 23 (VB33: ~- 2 - « , E «f:7 8, 7 9. 8 0 
If wSTiwk 2 2 5 3) 2 6 (W46B : ™* ^ 3) * »»» : 8 1 , 8 2. 8 
( (Sickl 7 2 a, 8 ' 29 ( ™ 8B:m ' ^ 3 > , Bm:.4. 8 6, 8 6 
MTO^ticDR!; 2 3 V 3 2 (VB11S : H » CDR1 - 2 ' 35 - : 3 7 , 8 8, 8 

TZ^kak 2, % 3 5 (VB14B: ~~ 2, 3, , 90 , 9 1,9 

( r 2 ]^,f!f^i 3 6 - 3 7 ' 38 <VB22B : HfflCDRl, 2, 3) , E?iJ#« -93 94 a 
5 (VB22B : LfflCDRl, 2, 3 ) «wjh"T ■ » J , a 4 . 9 

o 3 ^*"!/ 3 9 ~ 4 °~ 41 (VB16 : H«CDR1, 2, 3) , -9 6 9 7 9 

8 (VB16 : LttCDRl, 2, 3) mT 3 0 ' s ' - a 

ffiSHf 2 005-3004069 



#M 2004-071763 



^-v: 17/ 



(14) @2^!ll:-t : 4 2, 4 3 , 4 4 
1 0 1 (VB157 : LffltCDRl, 2, 3) 

(15) mzm-% : 4 5, 4 6 
^ 1 0 4 (VB4B : L&tCDRl, 

(16) ie?ij#^- : 4 8, 4 9 

> i o 7 (vB5i : lskcdri, 

(17) i5^J#^ :5 1, 5 2 

> 1 1 0 (AB317 

(18) @e?!]#-t : 5 

^ 1 1 3 (AB324 

(19) Se3W§-: 5 
^ 1 1 6 (TA136 

(20) @H^iJ#-^- : 2 



: LIKDR1, 
4, 5 5, 

: likdri, 

7, 5 8, 
: L&tCDRl, 



2, 
5 
2, 



(VB157 : HgJtCDRl, 2, 3) 
(VB4B : HilCDRl, 2, 3) 
HI1CDR1, 2, 3) 
: HiJtCDRl, 
: HiKDRl, 
H$JfCDRl, 



(VB51 : 
(AB317 



3 1 

4 4 

6 6 

7 7 

8 0 
> 2 



2 3 3 



, 4 7 
2, 3) 
5 0 
2, 3) 
5 3 
2, 3) 

5 6 (AB324 
3) 

9 (TA136 
3) 

2 3 5 (hVB22B p-z 



4 0 , 2 4 2 , 

(2D : 

7 3, 275, 
(22)@£?lJ#-4§- ; 
: 2 7 3 , 2 7 
[0 0 6 7] 
(VI) 



HStCDRl, 2, 3) 
HIJCDR1, 2, 3) 



2, 3) 
2, 3) 



2, 3) 



(hVB22B p-z : LgiCDRl, 2, 3) 
^ 2 6 8 , 2 7 0 (hVB22B g-e 
(hVB22B g-e : LIJtCDRl, 2, 3) 
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ffifiE# 2005-3004069 



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0. 4mM dNTPs (dATP, dGTP, dCTP, dTTP) , 

2. 5*.=.yh ODNA*° «; > 7 - -tfTaKaRa Ex Taq 

y 9 ^ m ^^^' ; ^^^^^ 22B -fP- sc-rLl5^c (± sc-fL15, 33 ■ 115LR ( 
[01781 

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0.4mM dNTPs (dATP, dGTP, dCTP, dTTP) 

2. M-^ ©una* * ? - -fe'TaKaRa Ex\aq 

l^LOfjl-pCRj^ (2««) , 
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[0 18 3] 17 -° 



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0.4mM dNTPs (dATP, dGTP, dCTP dTTP) 

2. ©UNA* U > 9 - -te'TaKaRa Ex^Taq 

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^l^ffiML*:^ pGEM-T Easy-?* * - (Promegatt*) n- - i * ur* 

GENM) *-fflv.T VnTrJZ J5Lu-J r QIAquxck Gel Extraction Kit (QIA 

^n-^ U PCXND3-VB22B sc(Fv)2^LA »3i^ * -pCXND3 

[0186] " 
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#©2004-071763 

^-v: 37/ 

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v»T450 n»^K*aE(itJI8655nB)t«iL 4^^?^^^^^^ Plus *« 

a^«^^ BaF3-human MpK B 

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sc(*02*fBRLfc o g ~ e * c N (Fv ^ PCXND3-hVB22B e 

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ffiSE#2 005-3004069 



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C^JfiM 3 ] AGSifcfc J: SitMpl^DiabodyOftSS 
**S ,^- n ! gr ° Wth selec tion)S (W003/91424#h?J Cfc $7=/-* n**fc**+ 
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3.1 I'hn^^^Xy^T'^,;^ 

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[0 2 0 0] 

PCRRfS»«(250^ L) «M^^[:^f 0 
25 //LO 10 X KOD Plus Buffer, 
25^Lfi02mM dNTPs (dATP, dGTP, dCTP dTTP) 
10//L<£>2.5mM MgS0 4 , 
7.5yuLO KOD Plus, 

[oTo 0 l l] m t ^ ttL<BO ™** K *»**mix primer 

98rc9MS^^T3^, 
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PCRSJS*«[ (100// L) Oftfig ^^tC^i- 0 
10//L«910XK0D Plus Buffer, 
10//L<?>2mM dNTPs (dATP, dGTP, dCTP dTTP) 
4//L?)2.5mM MgS0 4 , 
2 M L<D KOD Plus, 

[0 2 0 4] 

ffiiE# 2005-3004069 



#H 2004-071763 



^-v: 40/ 



25pmoL<^ sc-S£ Xf sc-AS £ ^ fln , 
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%KJc»S*7to QIAquick Gel Extraction Kit (QIAGEN?±§g) £Jfl v*t\ T^n-^^;i/^ 
fcffiS$*L7tPOig%$:>>*f ;^^^^-/7^^ KpMX/IL3ssGFPHis^SfiIffl5tt^}f AL 

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QIAGEN Plasmid Maxi Kit (QIAGENtt) *iv>Ty5^5 K^WttSL^o 
[0 2 0 7] 



tBSE# 2005-3004069 



#SS 2004-071763 



41/ 



H6l] 

f-*3-:178(HSl<4)) 
E?UM:179(HS2(4)) 
E*IJM:180(hS3 (3)) 
E5UM:181(HS4(4)) 
E$1JM:182(HS5 (7)) 
E5>]M:183<HS6 (2)) 
E5lJM:184(HS7 (l) ) 
E*UM:185<HS8(2)) 
186(HS9 (5) ) 
187(HS10 (2)) 
1B5IJM:188(HS11(2)) 
1B5IJ#^-:189(HS12 (2)) 
1B?'J§^-:190(HS13 (1)) 
E5»JM:191(hs14<2)) 
E5»JM:192(HS15<2)) 
E5>JM:193(HS16 (5)) 
E5>JM:194( H S17 (3.5) 
E$lJfH§-:195(HS18 (0.7)) 
E*)JM:196(HS19 (0.7 
BB5>J«:197(HA1(1)) 
E?iJM:198(HA2(D) 
E$»JM:199(HA3(:i)) 
E$UM:200(HA4(1)) 
EMM: 201 (LSI (l)) 
E$)JM:202OS2(2)) 
EMM:203 0.S3 (5)) 
5J5U§# : 204 (LS4 (3.5; 
E$UM:205Os5(4)) 
E$UM:206OS6(7)) 
E5UM:207 0,S7 (6)) 
EMM: 208<LSB (1.5) 

) 

:21O(LS10 (3.5 
M|-:211 (LSii(8)) 
2120S12 (8)) 



E51JM:209(LS9(2) 



EMM:2130,S13(6)) 
:214(LS14 (2)) 
:215(LS15 (2)) 
E5>JM:2160.S16(1)) 
E5>JM-: 2 1 7 (LS17 ( 1 ) ) 
E9J#^- : 2 1 8 Osiambde 
E?lJM:219(LAl(4)) 
E$1JM:2200.A2(4)) 
EMM: 221 (LA4 (4)) 
^:222(LA5 (4)) 
^:223(LAlambd; 



GCCCAGCCGGCCATGGCGGAKGTRMAGCTTCAGGAGTC 
GCCCAGCCGGCCATGGCGGAGGTBCAGCTBCAGCAGTC 
GCCCAGCCGGCCATGGCGCAGGTGCAGCTGAAGSASTC 
GCCCAGCCGGCCATGGCGGAGGTCCARCTGCAACARTC 
GCCCAGCCGGCCATGGCGCAGGTYCAGCTBCAGCARTC 
GCCCAGCCGGCCATGGCGCAGGTYCARCTGCAGCAGTC 
GCCCAGCCGGCCATGGCGCAGGTCCACGTGAAGCAGTC 
GCCCAGCCGGCCATGGCGGAGGTGAASSTGGTGGAATC 
GCCCAGCCGGCCATGGCGGAVGTGAWGYTGGTGGAGTC 
GCCCAGCCGGCCATGGCGGAGGTGCAGSKGGTGGAGTC 
GCCCAGCCGGCCATGGCGGAKGTGCAMCTGGTGGAGTC 
GCCCAGCCGGCCATGGCGGAGGTGAAGCTGATGGARTC 
GCCCAGCCGGCCATGGCGGAGGTGCARCTTGTTGAGTC 
GCCCAGCCGGCCATGGCGGARGTRAAGCTTCTCGAGTC 
GCCCAGCCGGCCATGGCGGAAGTGAARSTTGAGGAGTC 
GCCCAGCCGGCCATGGCGCAGGTTACTCTRAAAGWGTSTG 
) GCCCAGCCGGCCATGGCGCAGGTCCAACTVCAGCARCC 
') ) GCCCAGCCGGCCATGGCGGATGTGAACTTGGAAGTGTC 
f ) ) GCCCAGCCGGCCATGGCGGAGGTGAAGGTCATCGAGTC 
GGAGCCGCCGCCGCCCGAGGAAACGGTGACCGTGGT 
GGAGCCGCCGCCGCCCGAGGAGACTGTGAGAGTGGT 
GGAGCCGCCGCCGCCCGCAGAGACAGTGACCAGAGT 
GGAGCCGCCGCCGCCCGAGGAGACGGTGACTGAGGT 
GGCGGCGGCGGCTCCGAYATCCAGCTGACTCAGCC 
GGCGGCGGCGGCTCCGAYATTGTTCTCWCCCAGTC 
GGCGGCGGCGGCTCCGAYATTGTGMTMACTCAGTC 
) ) GGCGGCGGCGGCTCCGAYATTGTGYTRACACAGTC 
GGCGGCGGCGGCTCCGAYATTGTRATGACMCAGTC 
GGCGGCGGCGGCTCCGAYATTMAGATRAMCCAGTC 
GGCGGCGGCGGCTCCGAYATTCAGATGAYDCAGTC 
) GGCGGCGGCGGCTCCGAYATYCAGATGACACAGAC 
GGCGGCGGCGGCTCCGAYATTGTTCTCAWCCAGTC 
>) ) GGCGGCGGCGGCTCCGAYATTGWGCTSACCCAATC 
GGCGGCGGCGGCTCCGAYATTSTRATGACCCARTC 
GGCGGCGGCGGCTCCGAYRTTKTGATGACCCARAC 
GGCGGCGGCGGCTCCGAYATTGTGATGACBCAGKC 
GGCGGCGGCGGCTCCGAYATTGTGATAACYCAGGA 
GGCGGCGGCGGCTCCGAYATTGTGATGACCCAGWT 
GGCGGCGGCGGCTCCGAYATTGTGATGACACAACC 
GGCGGCGGCGGCTCCGAYATTTTGCTGACTCAGTC 
i(D) GGCGGCGGCGGCTCCGATGCTGTTGTGACTCAGGAATC 
GGAATTCGGCCCCCGAGGCCTTGATTTCCAGCTTGG 
GGAATTCGGCCCCCGAGGCCTTTATTTCCAGCTTGG 
GGAATTCGGCCCCCGAGGCCTTTATTTCCAACTTTG 
GGAATTCGGCCCCCGAGGCCTTCAGCTCCAGCTTGG 
1(D) GGAATTCGGCCCCCGAGGCCCCTAGGACAGT. 



CAGTTTGGI 



[0 2 0 8] 

3.2 AGSmzX&g&mfetfiowfr 



tBiiE#2 005-3004069 



#M 2 0 0 4 -0 7 1 7 6 3 ^- v ; 42/ 



cV» ; f ^~ y> ^* Pt - E ^orita^ Gene threapy vol 7 1063-106 
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lH*- fi % B FB i^^ tfDME ^(Invitrogentt^)-e6cm dishKJMU ^BFugeneltl 

f ^^^^^-^^#^#±ft^l0^g/mLO^y (Hexadime^H^ 

■* iOOOcells/ welHC4a«t-9 396well plate^lM L£ D 7BF B ^*^i+^a * 
[ 0 2 0 9^ -^DNA^rftttJ L, PCRffic J: Lfco 

KftRJ&igFft (50// L) OffijS *#:fcar$- 0 
5 M L<7) 10 X LA Taq Buffer, 
5^L<^2mM dNTPs (dATP, dGTP, dCTP, dTTP) 
5//L<7)2.5mM MgCU, 
0.5/liKD TaKaRa LA Taq. 

ADNA, 

2 ^ 2 le 1 ° 0 A J SdbS1 (K^J*-* : 2 2 6 ) , AGSdbAl (E?g*^ : 2 2 7 ) 

94TC/30»W, 60"C/3Qffr|Bj, 70<C/1^<7>^ * ^*30®K* 

*mf3R^»^72 < CT'6^»^L7t 0 
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1 5{K ^^^*SI^ j ?I;ab3 5 2 4 7 

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3. 3 AGSffiK «£ 19 # £ fL^DiabodyOfettfFffi 

*«WT^^5^J?!r? * ~ PCXND3 13 A L o DiabodyOS' *««*,; 

, ' GURU SffiMitSfeKLfc. 

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[fft 4 ] AB317fiCH0-mouse Mplfc«f^4 £ fcsWiB3*Lfc 0 

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[0216] 

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ffiiE#2 005-3004069 



2 004-071763 



43/ 



* StSunC8Ss?t U PC0S2 - MP ""' 'OOK. pC0S2-hMPLful 1C769T* 

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4.2 ^Mpl^ft#;Diabody % sc(Fv)20f^M 

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f 3 :^ ^^^aFS-human Mpl (G305C) |c*» t * 7 h ^T^^L A 

hsSSTV i a £ h Mpl * f ff P^^ B ^3-human Mpl (G305C) T?tt»^T^ 

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fctiE# 2005-3004069 



#H 2004-071763 



44/E 



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*2£f 1"^**° VE22B Diabod y<**>* AB317 Diabody(*«) t 4 fcC0S7«»Ji» 

[09] 0 9 j± % BaF-human Mpl£#v>fcAB324 DiabodyS i tfAB317 Diabody6D7=f 
2l2i^ feF-monkey Mpl £ffl^AB324 Diabody* <£ C/AB317 Diabody<D 
r?- il^iS^S^ 8 ^ 1 * 1 ^^ 24 Diabody^i^AB317 Diabody^ 
[012] 01211, hMPL-BaF3^#DiabodythTP0tWr*r=f-^ h 

[013] H13I1, hMPL(G305C)-BaF3^DiabodythTP0^^1-^,T=r^a hffiW 
^ti-C^^o 0.D.450/655nm£^U iat^g^^f, 

1 i ^ hMPL-B^m^^^t^TAlSe db, TA136 sc(Fv)2?>T rf^* h 
Stt*^0-e*4 o ffi»WtO.D.450/655nm*^U «ttt±zftft«-^-f 
4!^ 5tt hl«PL(G305C)-.BaF3*Bftfc:*»t*TA136 db, TAl36°sc(Fv)2G9 T 

=f --^ hjStt^^1-0T-*^ o 38*^0. D.450/655nm**U «8l 14 
4?i -2^ hMPL « ;76 9 T )-BaF3«B|&C*»t*TA136 db, TA136 sc(Fv)2<7>7 

^hpWl^^, 0 «tttiO.D.450/655nm**U **& 

4!^ ^LSi-2^^ L(C823A) - B ^ 3 '« }3jbnt * TA136 db. TA136 sc(Fv)2^T 
D h fS ^^l 8 ^**- «*ttO.D.450/655nm*:^L, »«^,7to 

R JS ^ 8 ?; * Hmmm < hVB22 B p-z:vh) Mbie^j (hv 

[ fJJ}JP 19 ^ V ^^M22B scCFV)2*J:OTiVB22B e sc(Fv)\ hVB22B g-e 
X$>Z> 0 m.m±m.±m. (450nm/655nm) fcj^U «ttl±ift£&jF-to 



mtE#2 005-3004069 



#M 2004-071763 ^ - : 

[mm] 

SEQUENCE LISTING 

<110> CHUGAI SEIYAKU KABUSHIKI KAISHA 

<120> anti-Mpl antibody 

<130> C1-A0320Y1 

<150> JP 2003-415746 
<151> 2003-12-12 

<160> 285 

<170> Patentln version 3.1 

<210> 1 

<211> 1572 

<212> DNA 

<213> Homo sapiens 

<400> 1 

atggactgga cctggaggtt cctctttgtg gtggcagcag ctacaggtgt ccagtcccag 60 

gtgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 120 

tgcaaggctt ctggatacac cttcaccaac tcctggatga actgggtgag gcagaggcct 180 

ggaaagggtc ttgagtggat gggacggatt tatcctggag atggagaaac tatctacaat 240 

gggaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 300 

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 360 

gattactcgt ttgcttactg gggccaggga accacggtca ccgtctcttc aggtggtggt 420 

ggatccggag gtggtggatc gggtggtgga ggatcggata ttgtgatgac tcagtctgca 480 

ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 540 

ctcctgcata gtaatggcaa cacttacttg tattggttcc agcagaagcc agggcagtct 600 

ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 660 

ggcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 720 

ggggtttatt actgcatgca acatatagaa tatcctttta cgttcggcca agggaccaaa 780 

ctggaaatca aaggaggtgg tggatcgggt ggtggtggtt cgggaggcgg tggatcgcag 840 

ffiiE# 2005-3004069 



#JS 2004-071763 ^- v : 2/ 

gtgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 900 

tgcaaggctt ctggatacac cttcaccaac tcctggatga actgggtgag gcagaggcct 960 

ggaaagggtc ttgagtggat gggacggatt tatcctggag atggagaaac tatctacaat 1020 

gggaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 1080 

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 1140 

gattactcgt ttgcttactg gggccaggga accacggtca ccgtctcttc aggtggtggt 1200 

ggatccggag gtggtggatc gggtggtgga ggatcggata ttgtgatgac tcagtctgca 1260 

ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 1320 

ctcctgcata gtaatggcaa cacttacttg tattggttcc agcagaagcc agggcagtct 1380 

ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 1440 

ggcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 1500 

ggggtttatt actgcatgca acatatagaa tatcctttta cgttcggcca agggaccaaa 1560 

ctggaaatca aa 2572 

<210> 2 

<211> 524 

<212> PRT 

<213> Homo sapiens 

<400> 2 

Met Asp Trp Thr Trp Arg Phe Leu Phe Val Val Ala Ala Ala Thr Gly 
1 5 10 15 

Val Gin Ser Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys 
20 25 30 

Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
35 40 45 

Thr Asn Ser Trp Met Asn Trp Val Arg Gin Arg Pro Gly Lys Gly Leu 
50 55 60 

Glu Trp Met Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 
65 70 75 80 

Gly Lys Phe Arg Val Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser 
85 90 95 

ftfE# 2005-3004069 



^12 0 04-071763 



^-v: 3/ 



Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
1Q 0 105 HO 

Tyr Tyr Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Glv 
115 120 125 

Gin Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 

135 140 

Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met Thr Gin Ser Ala 
I4i) 15 0 155 160 

Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser He Ser Cys Arg 
165 170 175 

Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp 
180 185 190 

Phe Gin Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu He Tyr Arg Met 
195 200 205 

Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
zw 215 220 

Gly Thr Ala Phe Thr Leu Lys He Ser Arg Val Glu Ala Glu Asp Val 
ZZb 23 0 235 240 

Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro Phe Thr Phe Gly 
245 250 255 

Gin Gly Thr Lys Leu Glu He Lys Gly Gly Gly Gly Ser Gly Gly Gly 
260 265 270 

Gly Ser Gly Gly Gly Gly Ser Gin Val Gin Leu Val Gin Ser Gly Pro 
275 280 285 

Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
MO 295 300 

Gly Tyr Thr Phe Thr Asn Ser Trp Met Asn Trp Val Arg Gin Arg Pro 
dUb 310 315 320 

Gly Lys Gly Leu Glu Trp Met Gly Arg He Tyr Pro Gly Asp Gly Glu 
325 330 335 

Thr He Tyr Asn Gly Lys Phe Arg Val Arg Val Thr He Thr Ala Asp 
340 345 350 

Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 

ffigE4#2 005-3004069 



#M 2004-071 763 



355 360 



365 



Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe 
U 37 5 380 

385 ^ ^ GIn £S ^ ^ Thr Val Ser Ser G1 * G ^ Gly 

390 395 4Q J 

Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp lie Val 
405 410 415 

Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser 



Met 



425 43 0 



He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr 
4 ^ 440 445 

Tyr Leu Tyr Trp Phe Gin Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu 

455 450 

lie Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 

470 475 4 8 o 

Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Lys He Ser Arg Val Glu 



490 495 

Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro 
500 505 510 

Phe Thr Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
515 520 



<210> 3 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 3 

Ser Ser Trp Met Asn 
1 5 



<210> 4 

<211> 17 

<212> PRT 

<213> Mus musculus 



4/ 



<400> 4 

Arg Thr Tyr P ro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 

ffi!E#2 005-3004069 



1 

Gly 



2004-071763 
10 



^-v : 5/ 



15 



<210> 5 

<211> 13 

<212> PRT 

<213> Mus musculus 

<400> 5 

Gly Trp He Leu Ala Asp Gly Gly Tyr Ser Phe Ala Tyr 
1 5 10 

<210> 6 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 6 

Ser Ser Trp Met Asn 
1 5 



<210> 7 

<211> 17 

<212> PRT 

<213> Mus musculus 



<400> 7 

Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 8 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 8 

Gly Tyr Ala Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 9 



ttifE# 2005-3004069 



#H 2004-071763 ^-ZS ; 6/ 



<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 9 

Ser Ser Tip Met Asn 
1 5 



<210> 10 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 10 

Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 11 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 11 

Gly Phe Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 12 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 12 

Ser Ser Trp Met Asn 
1 5 



<210> 13 

<211> 17 

<212> PRT 

<213> Mus musculus 



<400> 13 

Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

£BtE#2 005-3004069 



Gly 



#SI 2004-071763 



^-v: 7/ 



<210> 14 

<211> 9 

<212> PRT 

<213> Mus mus cuius 

<400> 14 

Gly Tyr Ala Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 15 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 15 

Arg Ser Trp Met Asn 
1 5 



<210> 16 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 16 

Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 17 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 17 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 18 
<211> 5 

ffiSE# 2005-3004069 



#M 2004-071763 

<212> PRT 

<213> Mus musculus 

<400> 18 

Asn Ser Trp Met Asn 
1 5 



<210> 19 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 19 

Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Asn Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 20 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 20 

Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 21 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 21 

Asn Tyr Trp Val Asn 
1 5 



<210> 22 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 22 

Arg He His Pro Ser Asp Ser Glu Thr His Cys Asn Gin Lys Phe Lys 
15 10 15 



tfJ!iE# 2005-3004069 



#® 2004-071763 



9/ 



Arg 



<210> 23 

<211> 6 

<212> PRT 

<213> Mus musculus 

<400> 23 

Gly Gly Trp Phe Ala Tyr 
1 5 



<210> 24 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 24 

Ser Ser Trp Met Asn 
1 5 



<210> 25 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 25 

Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Asn Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 26 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 26 

Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 27 
<211> 5 
<212> PRT 

tbtE#2 005-3004069 



#® 2004-071763 

<213> Mus musculus 



10/ 



<400> 27 

Thr Ser Trp Met Asn 
1 5 



<210> 28 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 28 

Arg He Tyr Pro Gly Asp Gly Glu Ala Asn Tyr Asn Gly Lys Phe Lys 
15 10 15 

Gly 



<210> 29 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 29 

Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 30 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 30 

Ser Ser Trp Met Asn 
1 5 



<210> 31 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 31 

Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 

£BSE#2 005-3004069 



#H2 004-071763 



^-P: 11/ 



<210> 32 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 32 

Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 33 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 33 

Arg Ser Trp Met Asn 
1 5 



<210> 34 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 34 

Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 35 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 35 

Gly Asp Gly Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 36 

<211> 5 

<212> PRT 

<213> Mus musculus 

ffi§E# 2005-3004069 



#® 2004-071763 



12/ 



<400> 36 

Asn Ser Trp Met Asn 
1 5 



<210> 37 

<211> 17 

<212> PRT 

<213> Mus musculus 



<400> 37 

Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe Arg 
1 5 10 15 



Val 



<210> 38 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 38 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 39 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 39 

Asp Tyr Trp Val Asn 
1 5 



<210> 40 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 40 

Arg He His Pro Tyr Asp Ser Glu Thr His Tyr Asn Gin Lys Phe Lys 
1 5 10 15 

Asn 



tB|E#2 005-3004069 



<210> 41 

<211> 6 

<212> PRT 

<213> Mus musculus 



12004-071763 



^-i> : 13/ 



<400> 41 

Gly Gly Trp Phe Ala Ser 
1 5 



<210> 42 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 42 

Asp Tyr Trp Met Asn 
1 5 



<210> 43 

<211> 17 

<212> PRT 

<213> Mus musculus 



<400> 43 

Arg He His Pro Phe Asp Ser Glu Thr His Cys Ser Gin Lys Phe Lys 
1 5 10 15 

Asn 



<210> 44 

<211> 6 

<212> PRT 

<213> Mus musculus 

<400> 44 

Gly Gly Trp Phe Ala Tyr 
1 5 



<210> 45 

<211> 5 

<212> PRT 

<213> Mus musculus 



miE#2 005-3004069 



<400> 45 

Asn Ser Trp Met Asn 
1 5 



#M 2004-071763 



^-v: 14/ 



<210> 


46 


<211> 


17 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


46 



Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe Arg 
1 5 10 15 

Val 



<210> 47 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 47 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 



1 


5 


<210> 


48 


<211> 


5 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


48 


Asn Ser Trp Met Asn 


1 


5 


<210> 


49 


<211> 


17 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


49 



Arg He Tyr Pro Gly Asp Gly Asp Thr He Tyr Asn Gly Asn Phe Lys 
1 5 10 15 

Gly 



ffi!E#2 005-3004069 



#M 2004-071763 



^-v: 15/ 



<210> 50 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 50 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 51 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 51 

Ser Tyr Thr Met Ser 
1 5 



<210> 52 

<211> 17 

<212> PRT 

<213> Mus musculus 




<210> 53 

<211> 6 

<212> PRT 

<213> Mus musculus 

<400> 53 

Arg Trp Phe Leu Asp Cys 
1 5 



<210> 54 

<211> 5 

<212> PRT 

<213> Mus musculus 

<400> 54 



(iJSE# 2005-3004069 



#M 2004-071763 



16/ 



Ser Ser Trp Met Asn 
1 5 



<210> 55 

<211> 17 

<212> PRT 

<213> Mus musculus 

<400> 55 

Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1 5 10 15 

Gly 



<210> 56 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 56 

Ala Arg Lys Thr Ser Trp Phe Ala Tyr 
1 5 



<210> 57 

<211> 6 

<212> PRT 

<213> Mus musculus 

<400> 57 

Ser Asp Tyr Ala Trp Ser 
1 5 



<210> 58 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 58 

Tyr He Thr Tyr Ser Gly Tyr Ser He Tyr Asn Pro Ser Leu Lys Ser 
15 10 15 



<210> 59 
<211> 7 
<212> PRT 



mfiE# 2005-3004069 



#H 2004-071763 

<213> Mus musculus 



^-v*: 17/ 



<400> 59 

Gly Tyr Asp Asn Met Asp Tyr 
1 5 



<210> 60 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 60 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 61 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 61 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 62 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 62 

Met Gin His Leu Glu Tyr Pro Phe Thr 
1 5 



<210> 63 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 63 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 64 
<211> 7 
<212> PRT 

ffiiE# 2005-3004069 



#0 2004-071763 

<213> Mus musculus 



^-v: 18/ 



<400> 64 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 65 

<211> 9 

•<212> PRT 

<213> Mus musculus 

<400> 65 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 

<210> 66 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 66 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 67 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 67 

Arg Met Ser Asn Leu Ala Ser 
1 5 

<210> 68 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 68 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 

<210> 69 
<211> 16 
<212> PRT 

&SE#2 0 0 5 - 3 0 0 4 0 6 9 



#H 2 0 0 4 - 0 7 1 7 6 3 v : 19/ 

<213> Mus musculus 
<400> 69 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
15 10 15 



<210> 70 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 70 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 71 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 71 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 72 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 72 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
15 10 15 



<210> 73 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 73 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 74 
<211> 9 
<212> PRT 



mSE#2 005-3004069 



#112 0 0 4 -0 7 1 7 6 3 . 2Q/ 

<213> Mus musculus 
<400> 74 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 75 

<211> 16 

<212> PRT 

<213> Mus musculus 



<400> 75 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 . 5 10 15 



<210> 76 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 76 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 77 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 77 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 78 

<211> 16 

<212> PRT 

<213> Mus musculus 



<400> 78 

Arg Ser Ser Lys Ser Leu Leu Tyr Ser Asn Gly Asn He Tyr Leu Tyr 
1 5 io 15 



<210> 79 
<211> 7 
<212> PRT 



miE# 2005-3004069 



#M 2 0 0 4 - 0 7 1 7 6 3 ^- v : 21/ 

<213> Mus musculus 
<400> 79 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 80 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 80 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 81 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 81 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 



<210> 82 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 82 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 83 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 83 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 84 
<211> 16 
<212> PRT 



2005-3004069 



#JH 2004-071763 

<213> Mus musculus 



^-v: 22/ 



<400> 84 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 85 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 85 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 86 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 86 

Met Gin His Val Glu Tyr Pro Tyr Thr 
1 5 



<210> 87 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 87 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 88 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 88 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 89 
<211> 9 
<212> PRT 

EBfiE# 2005-3004069 



#12 0 0 4-0 7 1 7 6 3 ^-v : 23/ 

<213> Mus musculus 



<400> 89 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 90 

<211> 16 

<212> PRT 

<213> Mus musculus 




<210> 91 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 91 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 92 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 92 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 93 

<211> 16 

<212> PRT 

<213> Mus musculus 



<400> 93 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 



15 



<210> 94 
<211> 7 
<212> PRT 



ffifE# 2005-3004069 



#12 0 0 4-0 7 1 7 6 3 : 24/ 



<213> Mus musculus 



<400> 94 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 95 

<211> 9 

<212> PRT 

<213> Mus musculus 



<400> 95 

Met Gin His He Glu Tyr Pro Phe Thr 
1 5 



<210> 96 

<211> 16 

<212> PRT 

<213> Mus musculus 




<210> 97 

<211> 7 

<212> PRT 

<213> Mus musculus 



<400> 97 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 98 

<211> 9 

<212> PRT 

<213> Mus musculus 



<400> 98 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 99 
<211> 16 
<212> PRT 



tB|E# 2005-3004069 



#©2004-071763 ^-v ; 25/ 



<213> Mus musculus 



<400> 99 

Arg Ser Ser Lys Ser Leu Leu Tyr Ser Asn Gly Asn He Tyr Leu Tyr 
1 5 iQ -- 



15 



<210> 100 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 100 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 101 

<211> 9 

<212> PRT 

<213> Mus musculus 



<400> 101 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 102 

<211> 16 

<212> PRT 

<213> Mus musculus 




<210> 103 

<211> 7 

<212> PRT 

<213> Mus musculus 



<400> 103 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 104 
<211> 9 
<212> PRT 



aiSE# 2005-3004069 



#M 2004-071763 

<213> Mus musculus 



^-v: 26/ 



<400> 104 

Met Gin His He Glu Tyr Pro Phe Thr 
1 5 



<210> 105 

<211> 16 

<212> PRT 

<213> Mus musculus 

<400> 105 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 

<210> 106 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 106 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 107 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 107 

Met Gin His Leu Glu Tyr Pro Tyr Thr 
1 5 



<210> 108 

<211> 15 

<212> PRT 

<213> Mus musculus 

<400> 108 

Arg Ala Ser Glu Ser Val Glu Tyr Tyr Gly Thr Ser Leu Met Gin 
1 5 10 15 

<210> 109 
<211> 7 
<212> PRT 

tBIE#2 005-3004069 



#M 2004-071763 

<213> Mus musculus 



•^-v : 27/ 



<400> 109 

Gly Ala Ser Asn Val Glu Ser 
1 5 



<210> 110 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 110 

Gin Gin Ser Arg Lys Val Pro Trp Thr 
1 5 

<210> 111 

<211> 11 

<212> PRT 

<213> Mus musculus 

<400> 111 

Lys Ala Ser Gin Asn Val Gly Asn He He Ala 
1 5 io 

<210> 112 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 112 

Leu Ala Ser Tyr Arg Tyr Ser 
1 5 



<210> 113 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 113 

Gin Gin Tyr Ser Ser Ser Pro Leu Thr 
1 5 



<210> 114 
<211> 12 
<212> PRT 

2005-3004069 



2004-071763 ^- >> • 28/ 

<213> Mus musculus 
<400> 114 

Ser Ala Ser Ser Ser Val Ser Ser Ser His Leu Tyr 
1 5 io 



<210> 115 

<211> 7 

<212> PRT 

<213> Mus musculus 

<400> 115 

Ser Thr Ser Asn Leu Ala Ser 
1 5 



<210> 116 

<211> 9 

<212> PRT 

<213> Mus musculus 

<400> 116 

His Gin Trp Ser Ser Tyr Pro Trp Thr 
1 5 



<210> 


117 


<211> 


411 


<212> 


DNA 


<213> 


Mus musculus 


<220> 




<221> 


CDS 


<222> 


(1).. (411) 


<223> 





<400> 117 

atg gaa tgg cct ttg ate ttt etc ttc etc ctg tea gga act gca ggt 
Met Glu Trp Pro Leu He Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
15 10 15 

gtc cac tec cag gtt cag ctg cag cag tct gga cct gag ctg gtg aag 
Val His Ser Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys 
20 25 30 

cct ggg gee tea gtg aag att tec tgc aag get tct ggc tat gca ttc 144 
Pro Gly Ala Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe 
35 40 45 



48 



96 



tB§JE#2 005-3004069 



#H 2 0 0 4 - 0 7 1 7 6 3 ^- v ; 29/ 

act aac tec tgg atg aac tgg gtg aag cag agg cct gga aag ggt ctt 192 
Thr Asn Ser Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu 
50 55 60 

gag tgg att gga egg att tat cct gga gat gga gaa act ate tac aat 240 
Glu Trp He Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 
65 70 75 80 

ggg aaa ttc agg gtc aag gee aca ctg act gca gac aaa tec tec age 288 
Gly Lys Phe Arg Val Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
85 90 95 

aca gee tac atg gat ate age age ctg aca tct gag gac tct gcg gtc 336 
Thr Ala Tyr Met Asp He Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
100 105 HO 

tac ttc tgt gca aga ggc tat gat gat tac teg ttt get tac tgg ggc 384 
Tyr Phe Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly 
H5 120 125 

caa ggg act ctg gtc act gtc tct gca 411 
Gin Gly Thr Leu Val Thr Val Ser Ala 
130 135 



<210> 118 

<211> 137 

<212> PRT 

<213> Mus musculus 

<400> 118 

Met Glu Trp Pro Leu He Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1 5 10 15 

Val His Ser Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys 
20 25 * 30 

Pro Gly Ala Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe 
35 40 45 

Thr Asn Ser Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu 
50 55 60 

Glu Trp He Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 
65 70 75 80 

Gly Lys Phe Arg Val Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
85 90 95 

Thr Ala Tyr Met Asp He Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 

l±JfiE#2 005-3004069 



#12 0 0 4 -0 7 1 7 6 3 : 30/ 



100 105 no 

Tyr Phe Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly 
115 120 125 



Gin Gly Thr Leu Val Thr Val Ser Ala 
130 135 



<210> 


119 


<211> 


396 


<212> 


DNA 


<213> 


Mus musculus 


<220> 




<221> 


CDS 


<222> 


(1).. (396) 


<223> 




<400> 


119 



m ? A gg ^ gC f ta gCt gag ttc ctg ggg ctg ctt Stg ttc tgg att cct 48 
Met Arg Cys Leu Ala Glu Phe Leu Gly Leu Leu Val Phe Trp He Pro 

15 10 15 

ff 3 ff° rf f 1 r\ l gtg Htg aCt cag gct gca ccc tct ata cct 96 
Gly Ala He Gly Asp He Val Met Thr Gin Ala Ala Pro Ser He Pro 

20 25 30 

p Ct rf ?f g t Ca gta tcC atc tcc tgt agg tct a gt aag agt 144 
Val Thr Pro Gly Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser 
35 40 45 

etc ctg cat agt aat ggc aac act tac ttg tat tgg ttc ctg cag agg 192 
Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg 
bU 55 60 

rf c Ct n Ct £f CtC Ctg ata tat cgg atg tc ^ aac ctt gec 240 
Pro Gly Gin Ser Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala 

W 70 75 80 

Ser Hv f* ^ 11° o gt ggC agt ggg tca gga act gct ttc 288 

Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe 

85 90 95 

?hr I^n To n° T f 3 f ? ?f g gCt gag gat gtg ggt gtt tat ta c 336 
Thr Leu Arg He Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr 

1°° 105 HO 

III Sp? nl T i\ a rf CCt tU acg ttc gga teg ggg acc aag 384 
Cys Met Gin His He Glu Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys 

tB|E# 2005-3004069 



#H 2004-071763 



^-v: 31/ 



115 



120 



125 



ctg gaa ata aaa 
Leu Glu He Lys 
130 



396 



<210> 120 

<211> 132 

<212> PRT 

<213> Mus musculus 

<400> 120 

Met Arg Cys Leu Ala Glu Phe Leu Gly Leu Leu Val Phe Trp He Pro 
1 5 10 15 

Gly Ala He Gly Asp He Val Met Thr Gin Ala Ala Pro Ser He Pro 
20 25 30 

Val Thr Pro Gly Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser 
35 40 45 

Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg 
50 55 60 

Pro Gly Gin Ser Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala 
65 70 75 80 

Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe 
85 90 95 

Thr Leu Arg He Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr 
100 105 110 

Cys Met Gin His He Glu Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys 
115 120 125 

Leu Glu He Lys 
130 



<210> 121 

<211> 762 

<212> DNA 

<213> Mus musculus 

<400> 121 

atggaatggc ctttgatctt tctcttcctc ctgtcaggaa ctgcaggtgt ccactcccag 60 



gttcagctgc agcagtctgg acctgagctg gtgaagcctg gggcctcagt gaagatttcc 120 

ffi!E# 2005-3004069 



#02 0 0 4 - 0 7 1 7 6 3 ^-v: 32/ 



180 



540 
600 
660 



tgcaaggctt ctggctatgc attcactaac tcctggatga actgggtgaa gcagaggcct 
ggaaagggtc ttgagtggat tggacggatt tatcctggag atggagaaac tatctacaat . 240 
gggaaattca gggtcaaggc cacactgact gcagacaaat cctccagcac agcctacatg 300 
gatatcagca gcctgacatc tgaggactct gcggtctact tctgtgcaag aggctatgat 360 
gattactcgt ttgcttactg gggccaaggg actctggtca ctgtctctgc aggtggtggt 420 
ggttcggata ttgtgatgac tcaggctgca ccctctatac ctgtcactcc tggagagtca 480 
gtatccatct cctgtaggtc tagtaagagt ctcctgcata gtaatggcaa cacttacttg 
tattggttcc tgcagaggcc aggccagtct cctcaactcc tgatatatcg gatgtccaac 
cttgcctcag gagtcccaga taggttcagt ggcagtgggt caggaactgc tttcacactg 
agaatcagta gagtggaggc tgaggatgtg ggtgtttatt actgtatgca acatatagaa 720 
tatcctttta cgttcggatc ggggaccaag ctggaaataa aa 762 

<210> 122 

<211> 254 

<212> PRT 

<213> Mus musculus 

<400> 122 

Met Glu Tip Pro Leu He Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1 . 5 10 15 

Val His Ser Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys 
20 25 30 

Pro Gly Ala Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe 
35 40 45 

Thr Asn Ser Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu 
DU 55 60 

Glu Trp He Gly Arg He Tyr Pro Gly Asp Gly Glu Thr lie Tyr Asn 

70 75 g0 

Gly Lys Phe Arg Val Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
85 90 95 

Thr Ala Tyr Met Asp lie Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
100 105 HO 

ffiiE#2 005-3004069 



#® 2004-071763 >? • 33/ 



Tyr Phe Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly 
115 120 125 

Gin Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Asp He 
130 135 140 

Val Met Thr Gin Ala Ala Pro Ser He Pro Val Thr Pro Gly Glu Ser 
145 150 155 160 

Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly 
165 170 175 

Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser Pro Gin 
180 185 190 

Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg 
195 200 205 

Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He Ser Arg 
210 215 220 

Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His He Glu 
225 230 235 240 

Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
245 250 



<210> 123 

<211> 635 

<212> PRT 

<213> Homo sapiens 

<400> 123 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu Leu Leu Ala 
15 10 15 

Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser Leu Leu Ala 
20 25 30 

Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe Glu Asp Leu 
35 40 45 

Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly Thr Tyr Gin 
50 55 60 

Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys Pro Leu Ser 
65 70 75 80 

mSE# 2005-3004069 



#M 2004-071763 

Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr Val Cys Gin Phe Pro 
85 90 95 

Asp Gin Glu Glu Val Arg Leu Phe Phe Pro Leu His Leu Trp Val Lys 
100 105 HO 

Asn Val Phe Leu Asn Gin Thr Arg Thr Gin Arg Val Leu Phe Val Asp 
115 120 . 125 

Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala Met Gly Glv 
130 135 HO 

Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu Glu Pro Ala Pro Glu 
145 150 155 160 

He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro Arg Asp Pro 
165 170 175 

Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala Thr Glu Thr 
180 185 190 

Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala Leu Asp Gin 
195 200 205 

Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly Pro Lys Gin 
21° 215 220 

Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu Gly Gly Ser 
225 230 235 240 

Cys Leu lie Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp Leu Gin Leu 
245 250 255 

Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp Gly Ser Trp 
260 265 270 

Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val Ala Leu Gly 
275 280 285 

Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys Gin Trp Gin 
2 90 295 300 

Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His Ser Arg Ala 
305 310 315 320 

Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn Cys Glu Glu 
325 330 335 

Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe Ser Arg Cys 
340 345 350 



^-v: 34/ 



ffiH#2 005-3004069 



#M 2004-071763 • 35/ 

His Phe Lys Ser Arg Asn Asp Ser He He His He Leu Val Glu Val 
355 360 365 

Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser Pro Phe Trp 
37 0 375 380 

He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His Trp Arg Glu 
385 390 395 400 

He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro Ser Ser Trp 
405 410 415 

Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly Glu Gly His 
420 425 430 

Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg Gly Gly Thr 
435 440 445 

Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu Arg Ala Arg 
450 455 460 

Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp Ser Asp Pro 
465 470 475 480 

Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser Leu Val Thr 
485 490 495 

Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly Leu Leu Leu 
500 505 510 

Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg His Ala Leu 
515 520 525 

Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin Tyr Leu Arg 
530 535 540 

Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser Asp Thr Cvs 
545 550 555 560 

Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys Ser Ser Glu 
565 570 575 

Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met Asp Tyr Arg 
580 585 590 

Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser Val Cys Pro 
595 600 605 

Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He Ala Asn His 

ffi!E# 2005-3004069 



4#M 2004-071763 
610 615 620 

Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 

630 635 



^-v: 36/ 



625 




<210> 


124 


<211> 


122 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


124 



Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
15 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg Thr Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Trp He Leu Ala Asp Gly Gly Tyr Ser Phe Ala Tyr Trp 
100 105 no 

Gly Gin Gly Thr Leu Val Thr Val Ser Ala 
115 120 



<210> 125 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 125 

Asp He Val Met Thr Gin Ala Ala Pro Ser He Pro Val Thr Pro Gly 
15 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 

£b§E#2 005-3004069 



#JH 2004-071763 



^-v: 37/ 



35 



40 



45 



Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly He Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Phe Thr Phe Gly Thr Gly Thr Lys Leu Glu He Lys 
100 105 110 



<210> 126 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 126 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
6 5 70 75 80 

He Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Tyr Ala Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 .o, 105 HO 

Leu Val Thr Val Ser Ala 
115 



<210> 127 

<211> 112 

<212> PRT 

<213> Mus musculus 



mtiE# 2005-3004069 



#H 2004-071763 



<400> 127 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 6Q 



' : 38/ 



Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg lie 
65 70 75 g 80 



Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu lie Lys 
100 105 ' no 

<210> 128 
<211> 118 
<212> PRT 
<213> Mus musculus 

<400> 128 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
15 10 15 

Ser Val Lys lie Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg lie Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
b5 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 go 95 

Ala Arg Gly Phe Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 HO 



ffiSE#2 005-3004069 



#M 2004-071763 



^-v: 39/ 



Leu Val Thr Val Ser Ala 
115 



<210> 129 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 129 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Ala Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Thr Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 110 

<210> 130 
<211> 118 
<212> PRT 
<213> Mus musculus 

<400> 130 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

&tE# 2005-3004069 



# gg 2004-071763 



Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Ser Gly Tyr Ala Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 110 

Leu Val Thr Val Ser Ala 
115 



<210> 131 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 131 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser lie Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 HO 



<210> 132 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 132 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
15 10 15 



^-v: 40/ 



mfE#2 005-3004069 



#M 2004-071763 



Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Arg Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Ser Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 no 

Leu Val Thr Val Ser Ala 
115 



<210> 133 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 133 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 io 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 HO 



^-v: 41/ 



tHM# 2005-3004069 



#M 2004-071763 



<210> 134 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 134 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Arg Ala Phe Gly Tyr Ala Phe Ser Asn Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Asn Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 no 

Leu Val Thr Val Ser Ala 
115 



^-v : 42/ 



<210> 135 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 135 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 



tfifE# 2005-3004069 



#M 2004-071763 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Ala Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 no 



<210> 136 

<211> 115 

<212> PRT 

<213> Mus musculus 

<400> 136 

Gin Val Gin Leu Gin Gin Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1 5 10 



^-i?: 43/ 



15 



Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
20 25 30 

Trp Val Asn Trp Val Lys Gin Arg Pro Gly Arg Gly Leu Glu Trp He 
35 40 45 

Gly Arg He His Pro Ser Asp Ser Glu Thr His Cys Asn Gin Lys Phe 
50 55 60 

Lys Arg Lys Ala Thr Leu Thr Val Asn Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

He Gin Leu His Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
85 90 95 

Thr Ser Gly Gly Trp Phe Ala Tyr Trp Gly Gin Gly Thr Leu Val Thr 
100 105 no 

Val Ser Ala 
115 



<210> 137 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 137 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
15 10 15 



ai§iE#2 005-3004069 



#M 2004-071763 ^- v : 44/ 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu Tyr Ser 
20 25 30 

Asn Gly Asn He Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 no 

<210> 138 
<211> 118 
<212> PRT 
<213> Mus musculus 

<400> 138 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Asn Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 no 

Leu Val Thr Val Ser Ala 
115 



£rjfE# 2005-3004069 



#1S 2 0 0 4- 0 7 1 7 6 3 ^- v : 45/ 

<210> 139 
<211> 112 
<212> PRT 
<213> Mus musculus 

<400> 139 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Ala Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 HO 



<210> 140 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 140 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 " 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Thr Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Ala Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Ser Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 

ttliE# 2005-3004069 



#M 2004-071763 



46/ 



85 



90 



95 



Ala Arg Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 110 



Leu Val Thr Val Ser Ala 
115 



<210> 


141 


<211> 


112 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


141 



Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Met Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Val Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 no 



<210> 142 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 142 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Pro Glu Trp He 

miE#2 005-3004069 



#M 2004-071763 



47/ 



35 



40 



45 



Gly Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Val Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Tyr Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 ' HO 

Leu Val Thr Val Ser Ala 
115 



<210> 


143 


<211> 


112 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


143 



Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
6 5 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 HO 



<210> 144 

<211> 118 

<212> PRT 

<213> Mus musculus 



i±JiE#2 005-3004069 



#SI 2004-071763 



<400> 144 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Leu Asn Pro Gly Ala 
1 5 10 is 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Arg Ser 
20 25 30 & 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Thr Asn Tyr Asn Gly Lys Phe 
50 55 60 

Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Thr Thr Ala Tyr 
65 70 75 80 

Met Gin Phe Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Asp Gly Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 HO 

Leu Val Thr Val Ser Ala 
115 



<210> 145 

<211> 112 

<212> PRT 

<213> Mus mus cuius 

<400> 145 

Asp lie Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 



: 48/ 



ffilE#2 005-3004069 



#M 2004-071763 



^-v: 49/ 



Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 110 



<210> 146 

<211> 115 

<212> PRT 

<213> Mus musculus 

<400> 146 

Gin Val Gin Leu Gin Gin Pro Gly Thr Glu Leu Val Arg Pro Gly Ala 
1 5 10 " 15 

Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
20 25 30 

Trp Val Asn Trp Val Lys Gin Arg Pro Gly Arg Gly Leu Glu Trp He 
35 40 45 

Gly Arg He His Pro Tyr Asp Ser Glu Thr His Tyr Asn Gin Lys Phe 
50 55 60 

Lys Asn Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

He Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
85 90 ^ 95 

Ala Ser Gly Gly Trp Phe Ala Ser Trp Gly Gin Gly Thr Leu Val Thr 
100 105 HO 

Val Ser Ala 
115 



<210> 


147 


<211> 


112 


<212> 


PRT 


<213> 


Mus musculus 


<400> 


147 



Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu Tyr Ser 
20 25 ' 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

ttiSE#2 005-3004069 



#® 2004-071763 



50/ 



Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Thr He 
65 70 75 80 

Ser Ser Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 HO 



<210> 148 

<211> 115 

<212> PRT 

<213> Mus musculus 

<400> 148 

Gin Val Gin Leu Gin Gin Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
20 25 30 

Trp Met Asn Trp Val Lys Gin Arg Pro Gly Arg Gly Leu Glu Trp He 
35 40 45 

Gly Arg He His Pro Phe Asp Ser Glu Thr His Cys Ser Gin Lys Phe 
50 55 60 

Lys Asn Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Asn Thr Ala Tyr 
65 70 75 80 

He Gin Phe Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
85 90 ' 95 

Ser Ser Gly Gly Trp Phe Ala Tyr Trp Gly Gin Gly Thr Leu Val Thr 
100 105 HO 

Val Ser Ala 
115 



<210> 149 

<211> 112 

<212> PRT 

<213> Mus musculus 



fcBIE#2 005-3004069 



#® 2004-071763 

<400> 149 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Ser Val Thr Pro Gly 
15 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu Tyr Ser 
20 25 30 

Asn Gly Asn He Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Lys He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 no 



<210> 150 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 150 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
15 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Ser 
20 25 30 

Trp Met Asn Trp Val Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe 
50 55 60 

Arg Val Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65 70 75 80 

Met Glu He Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 110 



: 51/ 



tfiiE# 2005-3004069 



2004-071763 



52/ 



Leu Val Thr Val Ser Ala 
115 



<210> 151 

<211> 112 

<212> PRT 

<213> Mus musculus 



<400> 151 

Asp He Val Met Thr Gin Ala Ala Pro Ser Val Pro Val Thr Pro Gly 
1 5 10 



15 



Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Asn 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 g0 



Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg IU 
65 70 75 8 go 



Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

He Glu Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
!00 105 no 



<210> 152 

<211> 118 

<212> PRT 

<213> Mus musculus 

<400> 152 

Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Asn Ser 
20 25 30 

Trp Met Asn Trp Val Asn Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Asp Thr He Tyr Asn Gly Asn Phe 
50 55 60 



ffiIE# 2005-3004069 



# M. 2004-071763 



Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser He Ala Tyr 
65 70 75 80 

Met Gin Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
85 90 95 

Thr Ser Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 110 

Leu Val Thr Val Ser Ala 
115 



<210> 153 

<211> 112 

<212> PRT 

<213> Mus musculus 

<400> 153 

Asp He Val Met Thr Gin Ala Ala Pro Ser Leu Pro Val Thr Pro Gly 
1.5 10 15 

Glu Ser Val Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser 
35 40 • 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

Leu Glu Tyr Pro Tyr Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
100 105 110 



<210> 


154 


<211> 


423 


<212> 


DNA 


<213> 


Mus musculus 


<220> 




<221> 


CDS 


<222> 


(1).. (423) 


<223> 





tBsEif#2 005-3004069 



#® 2004-071763 
<400> 154 

atg gtt ctt gcc age tct acc acc age ate cac ace atg ctg etc cte 48 
Met Val Leu Ala Ser Ser Thr Thr Ser He His Thr Met Leu Leu Leu 
1 5 10 15 

etc ctg atg ctg gcc cag ccg gcc atg gcg gaa gtg aag ctg gtg gag 
Leu Leu Met Leu Ala Gin Pro Ala Met Ala Glu Val Lys Leu Val Glu 
20 25 30. 

tct ggg gga ggc tta gtg aag cct gga ggg tec egg aaa etc tec tgt 
Ser Gly Gly Giy Leu Val Lys Pro Gly Gly Ser Arg Lys Leu Ser Cys 
35 40 45 

gca gcc tct gga ttc act ttc agt age tat acc atg tct tgg gtt cgc 
Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met Ser Trp Val Arg 
50 55 60 

cag act ccg gcg aag agg ctg gag tgg gtc gca acc att agt agt ggc 
Gin Thr Pro Ala Lys Arg Leu Glu Trp Val Ala Thr He Ser Ser Gly 
65 70 75 80 y 

agt agt acc ate tac tat gca gac aca gtg aag ggc cga ttc acc ate 
Ser Ser Thr He Tyr Tyr Ala Asp Thr Val Lys Gly Arg Phe Thr He 
85 90 ^ 95 

tec aga gac aat gcc aag aac acc ctg ttc ctg caa atg acc agt eta 
Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe Leu Gin Met Thr Ser Leu 
100 105 no 

agg tct gag gac aca gcc atg tat tac tgt gca agg aga tgg ttt ctt 
Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg Arg Trp Phe Leu 
115 120 125 

gac tgc tgg ggc caa ggc acc act etc aca gtc tec teg 
Asp Cys Trp Gly Gin Gly Thr Thr Leu Thr Val Ser Ser 
130 135 140 



<210> 155 

<211> 141 

<212> PRT 

<213> Mus mus cuius 

<400> 155 

Met Val Leu Ala Ser Ser Thr Thr Ser He His Thr Met Leu Leu Leu 
1 5 10 



^-v: 54/ 



15 



Leu Leu Met Leu Ala Gin Pro Ala Met Ala Glu Val Lys Leu Val Glu 
20 25 30 



96 



144 



192 



240 



288 



336 



384 



423 



ffiIE# 2005-3004069 



2004-071763 



Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Arg Lys Leu Ser Cys 
35 40 45 

Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met Ser Trp Val Arg 
50 55 60 

Gin Thr Pro Ala Lys Arg Leu Glu Trp Val Ala Thr He Ser Ser Glv 
65 70 75 go 

Ser Ser Thr He Tyr Tyr Ala Asp Thr Val Lys Gly Arg Phe Thr He 
85 90 ~ 95 

Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe Leu Gin Met Thr Ser Leu 
100 105 no 

Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg Arg Trp Phe Leu 
115 120 125 

Asp Cys Trp Gly Gin Gly Thr Thr Leu Thr Val Ser Ser 
13° 135 140 



^-v: 55/ 



<210> 


156 


<211> 


357 


<212> 


DNA 


<213> 


Mus musculus 


<220> 




<221> 


CDS 


<222> 


(1).. (357) 


<223> 




<400> 


156 



gat att gtg etc acc caa tct cca get tct ttg get gtg tct eta ggg 48 
Asp lie Val Leu Thr Gin Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1 5 10 15 

Hn ?? f? ?f C c CC ^ gC aga gCC agt gaa agt «" ^ aa tat tat 96 
Gin Ser Val Thr He Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr 

20 25 30 

ggc act agt tta atg cag tgg tac caa cag aaa cca gga cag cca ccc 144 
Gly Thr Ser Leu Met Gin Trp Tyr Gin Gin Lys Pro Gly Gin Pro Pro 
35 40 45 

aaa etc etc ate tat ggt gca tec aac gta gaa tct me. etc cct acc 1Q? 
Lys Leu Leu He Tyr Gly Ala Ser Asn Val Glu Ser ™ Val Fro All 
50 55 60 



tbfE# 2005-3004069 



12004-071763 



56/ 



aS ? gt ?f ?f g J Ct ggg aCa gaC ttc agc ctc aac at ^ cat 
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn He His 

65 70 75 80 

Prn {Ef n g ?f f 1 att gCa atg tat ttc ^ ca S caa a St agg 
Pro Val Glu Glu Asp Asp He Ala Met Tyr Phe Cys Gin Gin Ser Arg 

85 90 95 

aag gtt ccg tgg acg ttc ggt gga ggc acc aag ctg gaa ata aag gac 
Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Lei Glu He Lys fsp 
!00 105 no 

tac aag gat gac gac gat aag 
Tyr Lys Asp Asp Asp Asp Lys 
115 



240 



288 



336 



357 



<210> 157 

<211> 119 

<212> PRT 

<213> Mus musculus 

<400> 157 

Asp He Val Leu Thr Gin Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1 5 10 15 

Gin Ser Val Thr He Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr 
20 25 30 

Gly Thr Ser Leu Met Gin Trp Tyr Gin Gin Lys Pro Gly Gin Pro Pro 
35 40 45 

Lys Leu Leu He Tyr Gly Ala Ser Asn Val Glu Ser Gly Val Pro Ala 
50 55 60 

Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn He His 
bb 70 75 80 

Pro Val Glu Glu Asp Asp He Ala Met Tyr Phe Cys Gin Gin Ser Arg 
85 90 95 

Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu He Lys Asp 
!00 105 HO 

Tyr Lys Asp Asp Asp Asp Lys 
115 



<210> 158 
<211> 432 



ffitE4# 2005-3004069 



#H 2 0 0 4 - 0 7 1 7 6 3 : 57/ 

<212> DNA 

<213> Mus musculus 

<220> 

<221> CDS 
<222> (1)..(432) 
<223> 

<400> 158 

atg gtt ctt gcc age tct ace acc age ate cac ace atg ctg etc ctg 48 
Met Val Leu Ala Ser Ser Thr Thr Ser He His Thr Met Leu Leu Leu 
1 5-10 15 

etc ctg atg ctg gcc cag ccg gcc atg gcg cag gtt cag etc cag caa 96 
Leu Leu Met Leu Ala Gin Pro Ala Met Ala Gin Val Gin Leu Gin Gin 
20 25 30 

tct gga cct gag ctg gtg aag cct ggg gcc tea gtg aag att tec tgc 144 
Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys He Ser Cys 
35 40 45 

aag get tct ggc tat gca ttc agt age tec tgg atg aac tgg atg aag 192 
Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser Trp Met Asn Trp Met Lys 
50 55 60 

cag agg cct gga aag ggt ctt gag tgg att ggg egg att tat cct gga 240 
Gin Arg Pro Gly Lys Gly Leu Glu Trp He Gly Arg He Tyr Pro Gly 
65 70 75 " 80 

gat gga gat act aac tac aat ggg aag ttc aag ggc aag gcc aca ctg 288 
Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys Gly Lys Ala Thr Leu 
85 90 95 

act gca gac aaa tec tec age aca gcc tac atg caa etc age age ctg 336 
Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gin Leu Ser Ser Leu 
100 105 110 

aca tct gag gac tct gcg gtc tac ttc tgt gca aga gcg agg aaa act 384 
ihr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Ala Arg Lys Thr 
115 120 125 

tec tgg ttt get tac tgg ggc caa ggg act ctg gtc act gtc tct gcg 432 
Ser Trp Phe Ala Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ala 
130 135 140 

<210> 159 

<211> 144 

<212> PRT 

<213> Mus musculus 



mfE# 2005-3004069 



#H 2004-071763 *J ; 58/ 



<400> 159 

Met Val Leu Ala Ser Ser Thr Thr Ser He His Thr Met Leu Leu Leu 
1 5 10 15 

Leu Leu Met Leu Ala Gin Pro Ala Met Ala Gin Val Gin Leu Gin Gin 
20 25 30 

Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys He Ser Cys 
35 40 45 

Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser Trp Met Asn Trp Met Lys 
50 55 60 

Gin Arg Pro Gly Lys Gly Leu Glu Trp He Gly Arg He Tyr Pro Gly 
65 70 75 80 

Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys Gly Lys Ala Thr Leu 
85 90 95 

Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gin Leu Ser Ser Leu 
100 105 110 

Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Ala Arg Lys Thr 
115 120 125 

Ser Trp Phe Ala Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ala 
130 135 140 



<210> 160 

<211> 345 

<212> DNA 

<213> Mus musculus 

<220> 
<221> CDS 
<222> (1) . . (345) 
<223> 

<400> 160 

gac att gtg ttg aca cag tct caa aaa ttc atg tec aca tea gta gga 48 
Asp He Val Leu Thr Gin Ser Gin Lys Phe Met Ser Thr Ser Val Gly 
1 5 10 15 

gac agg gtc age ate age tgc aag gec agt cag aat gtg ggt aat att 96 
Asp Arg Val Ser He Ser Cys Lys Ala Ser Gin Asn Val Gly Asn He 
20 25 30 



gec tgg tat caa cag aaa cca ggg caa tct cct aaa gca ctg att 144 

tBBE#2 005-3004069 



#a 2004-071763 

He Ala Trp Tyr Gin Gin Lys Pro Gly Gin Ser Pro Lys Ala Leu He 
35 40 45 

tac ttg gca tec tac egg tac agt gga gtc cct gat cgc ttc aca ggc 192 
Tyr Leu Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
50 55 60 

agt gga tct ggg aca gat ttc act etc acc att agt aat gtg cag tct 240 
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr He Ser Asn Val Gin Ser 
65 70 75 80 

gaa gac ttg gca gag tat ttc tgt cag caa tat age age tct ccg etc 288 
Glu Asp Leu Ala Glu Tyr Phe Cys Gin Gin Tyr Ser Ser Ser Pro Leu 
85 90 95 

acg ttc ggt get ggg acc aag ctg gaa ata aag gac tac aag gat gac 336 
Thr Phe Gly Ala Gly Thr Lys Leu Glu He Lys Asp Tyr Lys Asp Asp 
100 105 HO 



: 59/ 



gac gat aag 
Asp Asp Lys 
115 



<210> 161 

<211> 115 

<212> PRT 

<213> Mus musculus 

<400> 161 

Asp He Val Leu Thr Gin Ser Gin Lys Phe Met Ser Thr Ser Val Gly 
1 5 10 15 

Asp Arg Val Ser He Ser Cys Lys Ala Ser Gin Asn Val Gly Asn He 
20 25 30 

He Ala Trp Tyr Gin Gin Lys Pro Gly Gin Ser Pro Lys Ala Leu He 
35 40 45 

Tyr Leu Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
50 55 60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr He Ser Asn Val Gin Ser 
65 70 75 80 

Glu Asp Leu Ala Glu Tyr Phe Cys Gin Gin Tyr Ser Ser Ser Pro Leu 
85 90 95 

Thr Phe Gly Ala Gly Thr Lys Leu Glu He Lys Asp Tyr Lys Asp Asp 
100 105 no 



345 



ffifE# 2005-3004069 



Asp Asp Lys 
115 



#M 2004-071763 



^-v: 60/ 



<210> 162 

<211> 116 

<212> PRT 

<213> Mus musculus 



<400> 162 

Asp Val Gin Leu Gin Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gin 



10 



15 



Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser He Thr Ser Asp 
20 25 30 

Tyr Ala Trp Ser Trp He Arg Gin Leu Pro Gly Asn Lys Leu Glu Trp 
JD 40 45 

Met Gly Tyr He Thr Tyr Ser Gly Tyr Ser He Tyr Asn Pro Ser 



55 60 



Leu 



Lys Ser Arg lie Ser lie Ser Arg Asp Thr Ser Lys Asn Gin Leu Phe 

70 75 go 

Leu Gin Leu Asn Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
85 90 95 

Val Gly Gly Tyr Asp Asn Met Asp Tyr Trp Gly Gin Gly Thr Ser Val 
100 105 110 

Thr Val Ser Ser 
115 



<210> 163 

<211> 108 

<212> PRT 

<213> Mus musculus 



<400> 163 

Gin He Val Leu Thr Gin Ser Pro Ala He Met Ser Ala Ser Pro Gly 



10 



15 



Glu Lys Val Thr Leu Thr Cys Ser Ala Ser Ser Ser Val Ser Ser Ser 
20 25 30 

His Leu Tyr Trp Tyr Gin Gin Lys Pro Gly Ser Ser Pro Lys Leu Trp 
35 40 45 



ffi!E#2 005-3004069 



#M 2004-071763 



He Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser 
50 55 60 

Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr He Ser Asn Met Glu 
65 70 75 80 

Thr Glu Asp Ala Ala Ser Tyr Phe Cys His Gin Tip Ser Ser Tyr Pro 
85 90 95 

Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu He Lys 
100 105 



<210> 164 
<211> 1924 
<212> DNA 

<213> Macaca fascicularis 
<220> 

<221> CDS 

<222> (11).. (1918) 

<223> 

<400> 164 

gaattccacc atg ccc tec tgg gec etc ttc atg gtc ace tec tgc etc 
Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu 
1 5 io 

etc ctg gee cct caa aac ctg gee caa gtc age age caa gat gtc tec 
Leu Leu Ala Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser 
15 20 25 

ttg ctg gee teg gac tea gag ccc ctg aag tgt ttc tec cga aca ttt 
Leu Leu Ala Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe 
30 35 40 45 



^-v: 61/ 



cag ttt cca gee cag gaa gaa gtg cgt etc ttc tct ccg ctg cac etc 



49 



97 



145 



gag gac etc act tgc ttc tgg gat gag gaa gag gca gca ccc agt ggg 193 
Glu Asp Leu Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly 
50 55 60 

aca tac cag ctg ctg tat gee tac ccg ggg gag aag ccc cgt gec tgc 241 
Thr Tyr Gin Leu Leu Tyr Ala Tyr Pro Gly Glu Lys Pro Arg Ala Cys 
65 70 75 

ccc ctg agt tct cag age gtg ccc cgc ttt gga acc cga tac gtg tgc 289 
Pro Leu Ser Ser Gin Ser Val Pro Arg Phe Gly Thr Arg Tyr Val Cys 
80 85 90 



337 



tiJIE# 2005-3004069 



#M 2004-071763 

Gin Phe Pro Ala Gin Glu Glu Val Arg Leu Phe Ser Pro Leu His Leu 
95 100 105 

tgg gtg aag aat gtg ttc eta aac cag act cag att cag cga gtc etc 
Trp Val Lys Asn Val Phe Leu Asn Gin Thr Gin He Gin Arg Val Leu 
110 H5 120 125 

ttt gtg gac agt gta ggc ctg ccg get ccc ccc agt ate ate aag gec 
Phe Val Asp Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala 
130 135 140 

atg ggt ggg age cag cca ggg gaa ctt cag ate age tgg gag gee cca 
Met Gly Gly Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu AU Pro 
145 150 155 



62/ 



ctg cag ctg cgc age gaa cct gat ggg ate tec etc ggt ggc tec tge 
Leu Gin Leu Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp 
255 260 265 



385 



433 



481 



Til P™ n! n C f ' f' ^ C T Ctg f g taC gaa Ctc cgc tat ^ c ccc 529 
Ala Pro Glu He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro 

160 165 170 

aaa gat etc aag aac tec act ggt ccc acg gtc ata cag ttg ate gee 577 
Lys Asp Leu Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala 
175 iso i 8 5 

?hr Pin ?h C ^ S Ct T Ctg C3g agg CCa Cac tca * cc tct * ct 625 
Thr Glu Thr Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala 

190 195 200 205 

ctg gac cag tct cca tgt get cag ccc aca atg ccc tgg caa gat gga 673 
Leu Asp Gin Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly 
210 215 220 

cca aag cag ace tec cca act aga gaa get tca get ctg aca gca gtg 721 
Pro Lys Gin Thr Ser Pro Thr Arg Glu Ala Ser Ala Leu Thr Ala Val 
225 230 235 

ggt gga age tge ctc ate tca gga ctc cag cct ggc aac tec tac tgg 769 
Gly Gly Ser Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Tro 
240 245 250 



817 



gga tec tgg tec ctc cct gtg act gtg gac ctg cct gga gat gca ata 865 
Gly Ser Trp Ser Leu Pro Val Thr Val Asp Leu Pro Gly £p Ala Val 
270 275 280 285 

gca att gga ctg caa tge ttt acc ttg gac ctg aag aat gtt acc tgt 913 
Ala He Gly Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys 
290 295 300 

ffifE# 2005-3004069 



mm 2 0 0 4 - 0 7 1 7 6 3 ^-v' : 63/ 

caa tgg cag caa gag gac cat get agt tec caa ggt ttc ttc tac cac 961 
Gin Trp Gin Gin Glu Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His 
305 310 315 

age agg gca egg tgc tgc ccc aga gac agg tac ccc ate tgg gag gac 1009 
Ser Arg Ala Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asd 
320 325 ~ ' 330 

tgt gaa gag gaa gag aaa aca aat cca gga tta cag ace cca cag ttc 1057 
Cys Glu Glu Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe 
335 340 345 

tct cgc tgc cac ttc aag tea cga aat gac age gtt att cac ate ctt 1105 
Ser Arg Cys His Phe Lys Ser Arg Asn Asp Ser Val He His He Leu 
350 355 360 3 65 

?f S £ tg aCC aCa gcc ctg ggt gct gtt cac a St tac ctg ggc tec 1153 
Val Glu Val Thr Thr Ala Leu Gly Ala Val His Ser Tyr Leu Gly Ser 

370 375 380 

cct ttc tgg ate cac cag gct gtg cgc etc ccc ace cca aac ttg cac 1201 
Pro Phe Trp He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His 
385 390 395 

tgg agg gag ate tec age ggg cat ctg gaa ttg gag tgg cag cac cca 1249 
Trp Arg Glu He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro 
400 405 410 

tea tec tgg gca gcc caa gag ace tgc tat caa etc cga tac aca gga 1297 
Ser Ser Trp Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Glv 
415 420 425 

gaa ggc cat cag gac tgg aag gtg ctg gag ccg cct etc ggg gcc cga 1345 
Glu Gly His Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg 
430 435 440 445 

?f ?f g 11° f tg ? ag Ctg CgC ccg cga tct cgc tac cgt tta cag ctg 1393 
Gly Gly Thr Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu 
450 455 460 

cgc gcc agg etc aat ggc ccc ace tac caa ggt ccc tgg age teg tgg 1441 
Arg Ala Arg Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp 
465 470 475 

teg gac cca gct agg gtg gag acc gcc acc gag ace gcc tgg att tec 1489 
Ser Asp Pro Ala Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser 
480 485 490 

ttg gtg acc gct ctg ctg eta gtg ctg ggc etc age gcc gtc ctg ggc 1537 

ffiSE#2 005-3004069 



<210> 165 

<211> 635 

<212> PRT 

<213> Macaca fascicularis 

<400> 165 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu Leu Leu Ala 
1 5 10 15 

Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser Leu Leu Ala 
20 25 30 



1585 



#M 2004-071763 

Leu Val Thr Ala Leu Leu Leu Val Leu Gly Leu Ser Ala Val Leu Gly 
495 500 505 

ctg ctg ctg ctg agg tgg cag ttt cct gca cac tac agg aga ctg agg 
Leu Leu Leu Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg 
510 515 520 ~ ~ 525 

cat gcc ctg tgg ccc tea ctt cca gat ctg cac cga gtc eta ggc cag 1633 
His Ala Leu Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin 
530 535 540 

tac ctt agg gac act gca gcc ctg agt ccg ccc aag gcc aca gtc tea 1681 
Tyr Leu Arg Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser 
545 550 555 

gat acc tgt gaa gaa gtg gaa ccc age etc ctt gaa ate etc ccc aag 1729 
Asp Thr Cys Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys 
560 565 570 

tec tea gag agg act cct ttg ccc ctg tgt tec tec cag tec cag atg 1777 
Ser Ser Glu Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ser Gin Met 
575 580 585 

gac tac cga aga ttg cag cct tct tgc ctg ggg acc atg ccc ctg tct 
Asp Tyr Arg Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser 
590 595 600 605 

gtg tgc cca ccc atg get gag tea ggg tec tgc tgt acc acc cac att 
Val Cys Pro Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He 
61 0 615 620 

gcc aac cat tec tac eta cca eta age tat tgg cag cag cct tga 

Ala Asn His Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 

gtcgac 



^-v: 64/ 



1825 



1873 



1918 



1924 



ffifE# 2005-3004069 



#® 2004-071763 

Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe Glu Asp Leu 
35 40 45 

Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly Thr Tyr Gin 
50 55 60 

Leu Leu Tyr Ala Tyr Pro Gly Glu Lys Pro Arg Ala Cys Pro Leu Ser 
65 70 75 80 

Ser Gin Ser Val Pro Arg Phe Gly Thr Arg Tyr Val Cys Gin Phe Pro 
85 90 95 

Ala Gin Glu Glu Val Arg Leu Phe Ser Pro Leu His Leu Trp Val Lys 
1Q 0 105 no 

Asn Val Phe Leu Asn Gin Thr Gin He Gin Arg Val Leu Phe Val Asp 
115 120 125 

Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala Met Gly Gly 
130 135 140 

Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu Ala Pro Ala Pro Glu 
145 150 155 160 

He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro Lys Asp Leu 
165 170 175 

Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala Thr Glu Thr 
180 185 190 

Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala Leu Asp Gin 
195 200 205 

Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly Pro Lys Gin 
^10 215 220 

Thr Ser Pro Thr Arg Glu Ala Ser Ala Leu Thr Ala Val Gly Gly Ser 
225 230 235 240 

Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp Leu Gin Leu 
245 250 255 

Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp Gly Ser Trp 
260 265 270 

Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val Ala He Gly 
275 280 285 

Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys Gin Trp Gin 
290 295 300 



^-v . 65/ 



£BiE#2 005-3004069 



2004-071763 66/ 



Gin Glu Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His Ser Arg Ala 
305 310 315 320 

Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asp Cys Glu Glu 
325 330 335 

Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe Ser Arg Cys 
340 345 350 

His Phe Lys Ser Arg Asn Asp Ser Val He His He Leu Val Glu Val 
355 360 365 

Thr Thr Ala Leu Gly Ala Val His Ser Tyr Leu Gly Ser Pro Phe Trn 
370 375 380 

He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His Trp Arg Glu 
385 390 395 400 

He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro Ser Ser Trp 
405 410 415 

Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly Glu Gly His 
420 425 430 

Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg Gly Gly Thr 
435 440 445 

Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu Arg Ala Arg 
450 455 460 

Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp Ser Asp Pro 
465 470 475 480 

Ala Arg Val Glu Thr Ala Thr Glu Thr Ala Trp lie Ser Leu Val Thr 
485 490 495 

Ala Leu Leu Leu Val Leu Gly Leu Ser Ala Val Leu Gly Leu Leu Leu 
500 505 510 

Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg His Ala Leu 
515 520 525 

Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin Tyr Leu Arg 
530 535 540 

Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser Asp Thr Cys 
545 550 555 560 

Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys Ser Ser Glu 

HlfE# 2005-3004069 



#11 2004-071763 



67/ 



565 



570 



575 



Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ser Gin Met Asp Tyr Arg 
580 585 590 

Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser Val Cys Pro 
595 600 605 

Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He Ala Asn His 
610 615 620 

Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 



625 



630 



635 



<210> 166 

<211> 24 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized sequence 

<400> 166 

caggggccag tggatagact gatg 24 



<210> 167 

<211> 23 

<212> DNA 

<213> Artificial 



<220> 

<223> an artificially synthesized sequence 
<400> 167 

gctcactgga tggtgggaag atg 



<210> 168 

<211> 30 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 168 

tagaattcca ccatggaatg gcctttgatc 30 

tb|E# 2005-3004069 



#H 2004-071763 



^-v : 68/ 



<210> 169 

<211> 56 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 169 ■ 

agcctgagtc atcacaatat ccgatccgcc tccacctgca gagacagtga ccagag 56 

<210> 170 

<211> 56 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 170 

actctggtca ctgtctctgc aggtggaggc ggatcggata ttgtgatgac tcaggc 56 

<210> 171 

<211> 60 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 171 

attgcggccg cttatcactt atcgtcgtca tccttgtagt cttttatttc cagcttggtc 60 

<210> 172 

<211> 8 

<212> PRT 

<213> Artificial 

<220> 

<223> an artificially synthesized FLAG tag sequence 
<400> 172 

Asp Tyr Lys Asp Asp Asp Asp Lys 
1 5 



m«E# 2005-3004069 



#® 2004-071763 

<210> 173 
<211> 85 
<212> DMA 
<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 173 

tagaattcca ccatggaatg gcctttgatc tttctcttcc tcctgtcagg aactgcaggt 
gtccactccc aggttcagct gcagc 

<210> 174 

<211> 82 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 174 

tggtcactgt ctctgcaggt ggtggtggtt cgggtggtgg tggttcgggt ggtggcggat 
cggatattgt gatgactcag gc 

<210> 175 

<211> 82 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 175 

tgagtcatca caatatccga tccgccacca cccgaaccac caccacccga accaccacca 60 
cctgcagaga cagtgaccag ag g 2 

<210> 176 

<211> 25 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 



^-v: 69/ 



60 
85 



60 
82 



fctiiE# 2005-3004069 



#M 2004-071763 

<400> 176 

caggttcagc tgcagcagtc tggac 

<210> 177 

<211> 81 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 177 

gctgcagctg aacctgcgat ccaccgcctc ccgaaccacc accacccgat ccaccacctc 
cttttatttc cagcttggtc c 

<210> 178 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 178 

gcccagccgg ccatggcgga kgtrinagctt caggagtc 

<210> 179 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 179 

gcccagccgg ccatggcgga ggtbcagctb cagcagtc 

<210> 180 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 



^-v ! 70/ 
25 



tBfE# 2005-3004069 



#@ 2004-071763 

<400> 180 

gcccagccgg ccatggcgca ggtgcagctg aagsastc 

<210> 181 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 181 

gcccagccgg ccatggcgga ggtccarctg caacartc 

<210> 182 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 182 

gcccagccgg ccatggcgca ggtycagctb cagcartc 

<210> 183 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 183 

gcccagccgg ccatggcgca ggtycarctg cagcagtc 

<210> 184 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 184 

gcccagccgg ccatggcgca ggtccacgtg aagcagtc 



#M 2004-071763 



<210> 185 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 185 

gcccagccgg ccatggcgga ggtgaasstg gtggaatc 

<210> 186 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 186 

gcccagccgg ccatggcgga vgtgawgytg gtggagtc 

<210> 187 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 187 

gcccagccgg ccatggcgga ggtgcagskg gtggagtc 

<210> 188 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 188 

gcccagccgg ccatggcgga kgtgcamctg gtggagtc 



2004-071763 

<210> 189 
<211> 38 
<212> DNA 
<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 189 

gcccagccgg ccatggcgga ggtgaagctg atggartc 

<210> 190 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 190 

gcccagccgg ccatggcgga ggtgcarctt gttgagtc 38 

<210> 191 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 191 

gcccagccgg ccatggcgga rgtraagctt ctcgagtc 38 

<210> 192 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 192 

gcccagccgg ccatggcgga agtgaarstt gaggagtc 38 



^-v: 73/ 



38 



<210> 193 
<211> 40 



ffiSE# 2005-3004069 



4#® 2004-071763 



74/ 



<212> DNA 
<213> Artificial 



<220> 

<223> an artificially synthesized primer sequence 
<400> 193 

gcccagccgg ccatggcgca ggttactctr aaagwgtstg 

<210> 194 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 194 

gcccagccgg ccatggcgca ggtccaactv cagcarcc 

<210> 195 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 195 

gcccagccgg ccatggcgga tgtgaacttg gaagtgtc 

<210> 196 

<211> 38 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 196 

gcccagccgg ccatggcgga ggtgaaggtc atcgagtc 

<210> 197 

<211> 36 

<212> DNA 

<213> Artificial 



40 



38 



38 



38 



ffi!E#2 005-3004069 



#H 2004-071763 

<220> 

<223> an artificially synthesized primer sequence 
<400> 197 

ggagccgccg ccgcccgagg aaacggtgac cgtggt 

<210> 198 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 198 

ggagccgccg ccgcccgagg agactgtgag agtggt 

<210> 199 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 199 

ggagccgccg ccgcccgcag agacagtgac cagagt 

<210> 200 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 200 

ggagccgccg ccgcccgagg agacggtgac tgaggt 

<210> 201 

<211> 35 

<212> DNA 

<213> Artificial 



<220> 



#M 2004-071763 

<223> an artificially synthesized primer sequence 
<400> 201 

ggcggcggcg gctccgayat ccagctgact cagcc 

<210> 202 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 202 

ggcggcggcg gctccgayat tgttctcwcc cagtc 

<210> 203 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 203 

ggcggcggcg gctccgayat tgtgmtmact cagtc 

<210> 204 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 204 

ggcggcggcg gctccgayat tgtgytraca cagtc 

<210> 205 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 



<Wm 2004-071763 

<400> 205 

ggcggcggcg gctccgayat tgtratgacm cagtc 

<210> 206 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 206 

ggcggcggcg gctccgayat tmagatramc cagtc 

<210> 207 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 207 

ggcggcggcg gctccgayat tcagatgayd cagtc 

<210> 208 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 208 

ggcggcggcg gctccgayat ycagatgaca cagac 

<210> 209 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 209 

ggcggcggcg gctccgayat tgttctcawc cagtc 



#M 2004-071763 



<210> 210 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 210 

ggcggcggcg gctccgayat tgwgctsacc caatc 

<210> 211 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 211 

ggcggcggcg gctccgayat tstratgacc carte 

<210> 212 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 212 

ggcggcggcg gctccgayrt tktgatgacc carac 

<210> 213 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 213 

ggcggcggcg gctccgayat tgtgatgacb cagkc 



#M 2004-071763 



<210> 214 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 214 

ggcggcggcg gctccgayat tgtgataacy cagga 

<210> 215 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 215 

ggcggcggcg gctccgayat tgtgatgacc cagwt 

<210> 216 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 216 

ggcggcggcg gctccgayat tgtgatgaca caacc 

<210> 217 

<211> 35 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 217 

ggcggcggcg gctccgayat tttgctgact cagtc 

<210> 218 
<211> 38 



2004-071763 

<212> DNA 
<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 218 

ggcggcggcg gctccgatgc tgttgtgact caggaatc 

<210> 219 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 219 

ggaattcggc ccccgaggcc ttgatttcca gcttgg 

<210> 220 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 220 

ggaattcggc ccccgaggcc tttatttcca gcttgg 

<210> 221 

<211> 36 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 221 

ggaattcggc ccccgaggcc tttatttcca actttg 

<210> 222 

<211> 36 

<212> DNA 

<213> Artificial 



#H 2004-071763 

<220> 

<223> an artificially synthesized primer sequence 
<400> 222 

ggaattcggc ccccgaggcc ttcagctcca gcttgg 

<210> 223 

<211> 39 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized primer sequence 
<400> 223 

ggaattcggc ccccgaggcc cctaggacag tcagtttgg 39 

<210> 224 

<211> 27 

<212>- DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized sequence 
<400> 224 

ttactcgcgg cccagccggc catggcg 27 

<210> 225 

<211> 17 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized sequence 
<400> 225 

ggaattcggc ccccgag 27 

<210> 226 

<211> 20 

<212> DNA 

<213> Artificial 

<220> 



: 81/ 



36 



ffiIE# 2005-3004069 



# M 2004-071763 

<223> an artificially synthesized sequence 
<400> 226 

tcacttacag gctctctact 

<210> 227 

<211> 20 

<212> DNA 

<213> Artificial 

<220> 

<223> an artificially synthesized sequence 
<400> 227 

caggtggggt ctt teat tec 



<210> 228 

<211> 354 

<212> DNA 

<213> Homo sapiens 

<400> 228 

caggtgeage tggtgcagtc tggacctgag gtgaagaagc ctggggcctc agtgaaggtc 60 

tectgeaagg cttctggata caccttcacc aactcctgga tgaactgggt gaggcagagg 120 

cctggaaagg gtcttgagtg gatgggaegg atttatcctg gagatggaga aactatctac 180 

aatgggaaat tcagggtcag agtcacgatt accgcggacg aatccacgag cacagcctac 240 

atggagctga geagectgag atctgaggac acggccgtgt attactgtgc gagaggctat 300 

gatgattact cgtttgctta ctggggccag ggaaccaegg tcaccgtctc ttca 354 

<210> 229 

<211> 118 

<212> PRT 

<213> Homo sapiens 

<400> 229 

Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Ser 
20 25 30 

Trp Met Asn Trp Val Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp Met 

ffilE#2 005-3004069 



#M 2004-071763 



83/ 



35 



40 



45 



Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe 
50 55 60 

Arg Val Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65 70 75 80 

Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
85 90 95 

Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
100 105 HO 



Thr Val Thr Val Ser Ser 
115 



<210> 


230 


<211> 


30 


<212> 


PRT 


<213> 


Homo 


<400> 


230 



Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
20 25 30 



<210> 231 

<211> 5 

<212> PRT 

<213> Homo sapiens 

<400> 231 

Asn Ser Trp Met Asn 

1 5 



<210> 232 

<211> 14 

<212> PRT 

<213> Homo sapiens 

<400> 232 

Trp Val Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp Met Gly 
1 5 10 



£BIE# 2005-3004069 



#M 2004-071763 



^-v: 84/ 



<210> 233 

<211> 17 

<212> PRT 

<213> Homo sapiens 




<210> 234 

<211> 32 

<212> PRT 

<213> Homo sapiens 



<400> 234 

Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr Met Glu 
15 10 15 

Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
20 25 30 



<210> 235 

<211> 9 

<212> PRT 

<213> Homo sapiens 

<400> 235 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 


236 


<211> 


11 


<212> 


PRT 


<213> 


Homo 


<400> 


236 


Trp Gly Gin 


1 





10 



<210> 237 
<211> 336 
<212> DNA 



£H§JE#2 005-3004069 



2004-071763 ^-y : 85/ 
<213> Homo sapiens 
<400> 237 

gatattgtga tgactcagtc tgcactctcc ctgcccgtca cccctggaga gccggcctcc 60 

atctcctgca ggtctagtaa gagtctcctg catagtaatg gcaacactta cttgtattgg 120 

ttccagcaga agccagggca gtctccacag ctcctgatct atcggatgtc caaccttgcc 180 

tcaggggtcc ctgacaggtt cagtggcagt ggatcaggca cagcttttac actgaaaatc 240 

agcagagtgg aggctgagga tgttggggtt tattactgca tgcaacatat agaatatcct 300 

tttacgttcg gccaagggac caaactggaa atcaaa 336 



<210> 238 

<211> 112 

<212> PRT 

<213> Homo sapiens 

<400> 238 

Asp He Val Met Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Gly 
1 5 10 15 

Glu Pro Ala Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Gin Gin Lys Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Lys He 
65 70 75 80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His 
85 90 95 

He Glu Tyr Pro Phe Thr Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
100 105 110 



<210> 239 

<211> 23 

<212> PRT 

<213> Homo sapiens 

<400> 239 

£BSE#2 005-3004069 



#M 2004-071763 

Asp He Val Met Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Glv 
1 5 10 15 

Glu Pro Ala Ser He Ser Cys 
20 



<210> 240 

<211> 16 

<212> PRT 

<213> Homo sapiens 

<400> 240 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 - 



^-v: 86/ 



15 



<210> 241 

<211> 15 

<212> PRT 

<213> Homo sapiens 

<400> 241 

Trp Phe Gin Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu He Tyr 
15 10 15 

<210> 242 

<211> 7 

<212> PRT 

<213> Homo sapiens 

<400> 242 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 243 

<211> 32 

<212> PRT 

<213> Homo sapiens 

<400> 243 

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr 
15 10 15 

Leu Lys He Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys 
20 25 30 * " 



ffilE# 2005-3004069 



#M 2004-071763 



^— v : 87/ 



<210> 244 

<211> 9 

<212> PRT 

<213> Homo sapiens 

<400> 244 

Met Gin His He Glu Tyr Pro Phe Thr 
1 5 



<210> 245 

<211> 10 

<212> PRT 

<213> Homo sapiens 

<400> 245 

Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
1 5 10 



<210> 246 
<211> 1924 
<212> DNA 
<213> Homo sapiens 

<400> 246 

gaattccacc atgccctcct gggccctctt catggtcacc tcctgcctcc tcctggcccc 60 

tcaaaacctg gcccaagtca gcagccaaga tgtctccttg ctggcatcag actcagagcc 120 

cctgaagtgt ttctcccgaa catttgagga cctcacttgc ttctgggatg aggaagaggc 180 

agcgcccagt gggacatacc agctgctgta tgcctacccg cgggagaagc cccgtgcttg 240 

ccccctgagt tcccagagca tgccccactt tggaacccga tacgtgtgcc agtttccaga 300 

ccaggaggaa gtgcgtctct tctttccgct gcacctctgg gtgaagaatg tgttcctaaa 360 

ccagactcgg actcagcgag tcctctttgt ggacagtgta ggcctgccgg ctccccccag 420 

tatcatcaag gccatgggtg ggagccagcc aggggaactt cagatcagct gggaggagcc 480 

agctccagaa atcagtgatt tcctgaggta cgaactccgc tatggcccca gagatcccaa 540 

gaactccact ggtcccacgg tcatacagct gattgccaca gaaacctgct gccctgctct 600 

gcagagacct cactcagcct ctgctctgga ccagtctcca tgtgctcagc ccacaatgcc 660 

ctggcaagat ggaccaaagc agacctcccc aagtagagaa gcttcagctc tgacagcaga 720 

ffiiE# 2005-3004069 



12 0 0 4-0 7 1 7 6 3 

gggtggaagc tgcctcatct caggactcca gcctggcaac tcctactggc tgcagctgcg 780 

cagcgaacct gatgggatct ccctcggtgg ctcctgggga tcctggtccc tccctgtgac 840 

tgtggacctg cctggagatg cagtggcact tggactgcaa tgctttacct tggacctgaa 900 

gaatgttacc tgtcaatggc agcaacagga ccatgctagc tcccaaggct tcttctacca 960 

cagcagggca cggtgctgcc ccagagacag gtaccccatc tgggagaact gcgaagagga 1020 

agagaaaaca aatccaggac tacagacccc acagttctct cgctgccact tcaagtcacg 1080 

aaatgacagc attattcaca tccttgtgga ggtgaccaca gccccgggta ctgttcacag 1140 

ctacctgggc tcccctttct ggatccacca ggctgtgcgc ctccccaccc caaacttgca 1200 

ctggagggag atctccagtg ggcatctgga attggagtgg cagcacccat cgtcctgggc 1260 

agcccaagag acctgttatc aactccgata cacaggagaa ggccatcagg actggaaggt 1320 

gctggagccg cctctcgggg cccgaggagg gaccctggag ctgcgcccgc gatctcgcta 1380 

ccgtttacag ctgcgcgcca ggctcaacgg ccccacctac caaggtccct ggagctcgtg 1440 

gtcggaccca actagggtgg agaccgccac cgagaccgcc tggatctcct tggtgaccgc 1500 

tctgcatcta gtgctgggcc tcagcgccgt cctgggcctg ctgctgctga ggtggcagtt 1560 

tcctgcacac tacaggagac tgaggcatgc cctgtggccc tcacttccag acctgcaccg 1620 

ggtcctaggc cagtacctta gggacactgc agccctgagc ccgcccaagg ccacagtctc 1680 

agatacctgt gaagaagtgg aacccagcct ccttgaaatc ctccccaagt cctcagagag 1740 

gactcctttg cccctgtgtt cctcccaggc ccagatggac taccgaagat tgcagccttc 1800 

ttgcctgggg accatgcccc tgtctgtgtg cccacccatg gctgagtcag ggtcctgctg 1860 

taccacccac attgccaacc attcctacct accactaagc tattggcagc agccttgagt 1920 
cgac 



1924 



<210> 247 

<211> 1924 

<212> DNA 

<213> Homo sapiens 

<220> 

<221> CDS 



tBSE#2 005-3004069 



#02 0 0 4 - 0 7 1 7 6 3 ^-v : 89/ 



<222> (11).. (1918) 
<223> 



<400> 247 

gaattccacc atg ccc tec tgg gec etc ttc atg gtc ace tec tgc etc 
Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu 
1 5 10 



49 



etc ctg gee cct caa aac ctg gee caa gtc age age caa gat gtc tec 97 
Leu Leu Ala Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser 
15 20 25 

ttg ctg gca tea gac tea gag ccc ctg aag tgt ttc tec cga aca ttt 145 
Leu Leu Ala Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe 
30 35 40 ~ 45 

gag gac etc act tgc ttc tgg gat gag gaa gag gca gcg ccc agt ggg 193 
Glu Asp Leu Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly 
50 55 60 

aca tac cag ctg ctg tat gee tac ccg egg gag aag ccc cgt get tgc 241 
Thr Tyr Gin Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys 
65 70 75 

ccc ctg agt tec cag age atg ccc cac ttt gga ace cga tac gtg tgc 289 
Pro Leu Ser Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr Val Cys 
80 85 90 

cag ttt cca gac cag gag gaa gtg cct etc ttc ttt ccg ctg cac etc 337 
Gin Phe Pro Asp Gin Glu Glu Val Pro Leu Phe Phe Pro Leu His Leu 
95 100 105 

tgg gtg aag aat gtg ttc eta aac cag act egg act cag cga gtc etc 385 
Trp Val Lys Asn Val Phe Leu Asn Gin Thr Arg Thr Gin Arg Val Leu 
HO 115 120 " 125 

ttt gtg gac agt gta ggc ctg ccg get ccc ccc agt ate ate aag gee 433 
Phe Val Asp Ser Val Gly Leu Pro Ala Pro Pro Ser lie He Lys Ala 
130 135 140 

atg ggt ggg age cag cca ggg gaa ctt cag ate age tgg gag gag cca 481 
Met Gly Gly Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu Glu Pro 
145 150 155 

get cca gaa ate agt gat ttc ctg agg tac gaa etc cgc tat ggc ccc 529 
Ala Pro Glu He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro 
160 165 170 

aga gat ccc aag aac tec act ggt ccc acg gtc ata cag ctg att gec 577 
Arg Asp Pro Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala 

tBIE# 2005-3004069 



2004-071763 



90/ 



175 



180 



185 



ri a ?f r gC l gC S Ct gct ctg ca & a ^ a cct cac tc * gec tct get 625 
Thr Glu Thr Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala 

190 19 5 200 205 

ctg gac cag tct cca tgt gct cag ccc aca atg ccc tgg caa gat gga 673 
Leu Asp Gin Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly 
210 215 220 

cca aag cag acc tec cca agt aga gaa gct tea gct ctg aca gca gag 721 
Pro Lys Gin Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu 
225 230 235 

ggt gga age tgc etc ate tea gga etc cag cct ggc aac tec tac tgg 769 
Gly Gly Ser Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp 
240 245 250 

ctg cag ctg cgc age gaa cct gat ggg ate tec etc ggt ggc tec tgg 817 
Leu Gin Leu Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp 
255 260 265 

ff ^ CC i gg £ CC T ctc 5 ct Stg act gtg gac ctg cct gga gat gca gtg 865 
Gly Ser Trp Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val 

270 27 5 . 280 " " 285 

gca ctt gga ctg caa tgc ttt acc ttg gac ctg aag aat gtt acc tgt 913 
Ala Leu Gly Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys 
290 295 300 

caa tgg cag caa cag gac cat gct age tec caa ggc ttc ttc tac cac 961 
Gin Trp Gin Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His 
305 310 315 

age agg gca egg tgc tgc ccc aga gac agg tac ccc ate tgg gag aac 1009 
Ser Arg Ala Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn 
320 325 330 

tgc gaa gag gaa gag aaa aca aat cca gga eta cag acc cca cag ttc 1057 
Cys Glu Glu Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe 
335 340 345 

tct cgc tgc cac ttc aag tea cga aat gac age att att cac ate ctt 1105 
Ser Arg Cys His Phe Lys Ser Arg Asn Asp Ser He lie His He Leu 
350 355 360 365 

gtg gag gtg acc aca gee ccg ggt act gtt cac age tac ctg ggc tec 1153 
Val Glu Val Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser 
370 375 380 



&iE#2 005-3004069 



#M 2004-071763 



^-y: 91/ 



cct ttc tgg ate cac cag get gtg cgc etc ccc acc cca aac ttg cac 1201 
Pro Phe Trp He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His 
385 390 395 

tgg agg gag ate tec agt ggg cat ctg gaa ttg gag tgg cag cac cca 1249 
Trp Arg Glu He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro 
400 405 410 

teg tec tgg gca gee caa gag acc tgt tat caa etc cga tac aca gga 1297 
Ser Ser Trp Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Glv 
415 420 425 

rf rf S at ?? g f C tgg aag gtg Ctg gag ccg cct ctc Sgg gee cga 1345 
Glu Gly His Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg 

430 435 440 445 

gga ggg acc ctg gag ctg cgc ccg cga tct cgc tac cgt tta cag ctg 1393 
Gly Gly Thr Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu 
450 455 460 

cgc gee agg ctc aac ggc ccc acc tac caa ggt ccc tgg age teg tgg 1441 
Arg Ala Arg Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp 
465 470 475 

teg gac cca act agg gtg gag acc gec acc gag acc gec tgg ate tec 1489 
Ser Asp Pro Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser 
480 485 490 

T ttg S tg « c gCt Ctg cat cta gtg ctg ggc ctc a ^ c sec gtc ctg ggc 1537 
Leu Val Thr Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly 
495 500 505 

ctg ctg ctg ctg agg tgg cag ttt cct gca cac tac agg aga ctg agg 1585 
Leu Leu Leu Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg 
510 515 520 " 525 

cat gee ctg tgg ccc tea ctt cca gac ctg cac egg gtc cta ggc cag 1633 
His Ala Leu Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin 
530 535 540 

tac ctt agg gac act gca gee ctg age ccg ccc aag gee aca gtc tea 1681 
Tyr Leu Arg Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser 
545 550 555 

gat acc tgt gaa gaa gtg gaa ccc age ctc ctt gaa ate ctc ccc aag 1729 
Asp Thr Cys Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys 
560 565 570 

tec tea gag agg act cct ttg ccc ctg tgt tec tec cag gec cag atg 1777 
Ser Ser Glu Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met 

ffiaE#2 005-3004069 



#M 2004-071763 
575 580 585 



: 92/ 



? C J* 3 T Ug ^ g CCt tCt tgC ctg ggg acc at * ccc ct« tct 1825 
Asp Tyr Arg Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser 

590 5 95 600 605 

gtg tgc cca ccc atg get gag tea ggg tec tgc tgt acc acc cac att 
Val Cys Pro Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He 
610 615 620 

gcc aac cat tec tac eta cca eta age tat tgg cag cag cct tga 
Ala Asn His Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 



gtcgac 



<210> 248 

<211> 635 

<212> PRT 

<213> Homo sapiens 

<400> 248 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu Leu Leu Ala 
1 5 10 15 

Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser Leu Leu Ala 
20 25 30 

Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe Glu Asp Leu 
35 40 " 45 

Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly Thr Tyr Gin 
50 55 60 

Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys Pro Leu Ser 
65 70 75 go 

Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr Val Cys Gin Phe Pro 
85 90 95 

Asp Gin Glu Glu Val Pro Leu Phe Phe Pro Leu His Leu Trp Val Lys 
!00 105 HO 

Asn Val Phe Leu Asn Gin Thr Arg Thr Gin Arg Val Leu Phe Val Asp 
H5 120 125 

Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala Met Gly Gly 
idU 135 140 



1873 



1918 



1924 



ffilE# 2005-3004069 



#M 2004-071763 

Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu Glu Pro Ala Pro Glu 
145 150 155 lg0 

He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro Arg Asp Pro 
165 170 175 

Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala Thr Glu Thr 
180 185 190 

Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala Leu Asp Gin 
195 200 205 

Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly Pro Lys Gin 
210 215 220 

Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu Gly Gly Ser 
225 230 235 240 

Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp Leu Gin Leu 
245 250 255 

Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp Gly Ser Tro 
260 265 270 

Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val Ala Leu Gly 
275 280 285 

Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys Gin Trp Gin 
290 295 300 

Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His Ser Arg Ala 
305 310 315 320 

Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn Cys Glu Glu 
325 330 335 

Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe Ser Arg Cys 
340 345 350 

His Phe Lys Ser Arg Asn Asp Ser He He His He Leu Val Glu Val 
355 360 3 6 5 

Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser Pro Phe Trp 
370 375 3 8 o 

He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His Trp Arg Glu 
^ 5 390 395 400 

He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro Ser Ser Trp 
405 410 415 



^-v : 93/ 



ffiSE# 2005-3004069 



#M 2004-071763 



^-v: 94/ 



Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly Glu Gly His 
420 425 



430 



Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg Gly Gly Thr 
435 440 445 

Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu Arg Ala Arg 
t)U 455 460 

Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp Ser Asp Pro 



470 



475 



480 



Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp lie Ser Leu Val Thr 
485 490 495 

Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly Leu Leu Leu 
500 505 510 

Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg His Ala Leu 
515 520 525 

530 UU ^ HiS Arg Val LeU Gly Gln Tyr Leu Arg 



535 



540 



Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser Asp Thr Cys 



550 



555 560 
Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys Ser Ser Glu 



565 



570 



575 



Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met Asp Tyr Arg 
580 585 



590 

Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser Val Cys Pro 
595 600 605 

Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He Ala Asn His 
610 615 



620 



Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 



<210> 249 

<211> 1924 

<212> DNA 

<213> Homo sapiens 

<220> 



£tiiE# 2005-3004069 



#12 00 4-07 1763 



<221> CDS 

<222> (11).. (1918) 

<223> 



<400> 249 

gaattccacc atg ccc tec tgg gec etc ttc atg gtc acc tee tge etc 
Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu 
1 5 



10 



etc ctg gee cct caa aac ctg gee caa gtc age age caa gat gtc tec 
Leu Leu Ala Pro Gin Asn Leu Ala Gin Val Ser Ser Gin fsp f a l Ser 
io 20 25 

ttg ctg gca tea gac tea gag ccc ctg aag tgt ttc tec cea 3 r. f 
Leu Leu Ala Ser Asp Ser Glu Pro Leu Lyl Cys Phe Sel A?g p£e 
U 35 40 45 

gag gac etc act tgc ttc tgg gat gag gaa gag gca gcg ccc agt Sss 
Glu Asp Leu Thr Cys Phe Trp Asp Glu Glu Glu Ala Alf Pro Ser G?y 
50 55 go 



155 



: 95/ 



49 



97 



145 



193 



?hr rlr Pin t g i 8 J at ?? C taC CCg Cgg ^ aa * ccc cgt get tgc 241 
Thr Tyr Gin Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys 

65 70 75 

ccc ctg agt tec cag age atg ccc cac ttt gga acc cga tac sts tec ?SQ 
Pro Leu Ser Ser Gin Ser Met Pro His Phe Gly Thr A?g Tyr £f Cyt 



337 



385 



85 90 

S° a f ° ^ g gag gaa gtg c ^ c tc ttc ttt ccg ctg cac etc 
Gin Phe Pro Asp Gin Glu Glu Val Arg Leu Phe Phe Pro Leu His LeS 

y0 1Q 0 105 

tgg gtg aag aat gtg ttc eta aac cag act egg act cag cga gtc etc 
Trp Val Lys Asn Val Phe Leu Asn Gin Thr a£ Thr Gin A?g *? Leu 

115 120 12 5 

£ Sf 5 SS g S 2 S S S £5 £ £ £ 5 s « 

130 135 140 

a tg ggt ggg age cag cca ess zaa rtf mo o™- + 

Met Gl, G, y Ser Gin P ro l? 7 IT, £ ^ it Ser & £ SS £ 481 
145 150 " ' ~~ 



E S | £ 55 S £ 2 Arg £ SS £ 5 £ £ S » 

60 165 170 

aga gat ccc aag aac tee act ggt cee acg gtc ata cag ctg att gee 577 

ffiSE#2 005-3004069 



#® 2004-071763 t-z? : 96/ 

Arg Asp Pro Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala 
175 180 185 

aca gaa acc tgc tgc cct get ctg cag aga cct cac tea gee tct get 625 
Thr Glu Thr Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala 
190 195 200 205 

ctg gac cag tct cca tgt get cag ccc aca atg ccc tgg caa gat gga 673 
Leu Asp Gin Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly 
210 215 220 

cca aag cag acc tec cca agt aga gaa get tea get ctg aca gca gag 721 
Pro Lys Gin Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu 
225 230 235 

ggt gga age tgc etc ate tea gga etc cag cct ggc aac tec tac tgg 769 
Gly Gly Ser Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp 
240 245 250 

ctg cag ctg tgc age gaa cct gat ggg ate tec etc ggt ggc tec tgg 817 
Leu Gin Leu Cys Ser Glu Pro Asp Gly lie Ser Leu Gly Gly Ser Trp 
255 260 265 

gga tec tgg tec etc cct gtg act gtg gac ctg cct gga gat gca gtg 865 
Gly Ser Trp Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val 
270 275 280 * 285 

gca ctt gga ctg caa tgc ttt acc ttg gac ctg aag aat gtt acc tgt 913 
Ala Leu Gly Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys 
290 295 300 

caa tgg cag caa cag gac cat get age tec caa ggc ttc ttc tac cac 961 
Gin Trp Gin Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His 
305 310 315 

age agg gca egg tgc tgc ccc aga gac agg tac ccc ate tgg gag aac 1009 
Ser Arg Ala Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn 
320 325 330 

tgc gaa gag gaa gag aaa aca aat cca gga eta cag acc cca cag ttc 1057 
Cys Glu Glu Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe 
335 340 345 

tct cgc tgc cac ttc aag tea cga aat gac age att att cac ate ctt 1105 
Ser Arg Cys His Phe Lys Ser Arg Asn Asp Ser He He His He Leu 
350 355 3 6 o 365 

gtg gag gtg acc aca gec ccg ggt act gtt cac age tac ctg ggc tec 1153 
Val Glu Val Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser 
370 375 380 

ffilE# 2005-3004069 



#H 2004-071763 



^-v: 97/ 



cct ttc tgg ate cac cag get gtg cgc etc ccc acc cca aac ttg cac 1201 
Pro Phe Trp He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His 
385 390 395 

tgg agg gag ate tec agt ggg cat ctg gaa ttg gag tgg cag cac cca 1249 
Trp Arg Glu He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro 
400 405 410 

teg tec tgg gca gee caa gag acc tgt tat caa etc cga tac aca gga 1297 
Ser Ser Trp Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly 
415 420 425 

gaa ggc cat cag gac tgg aag gtg ctg gag ccg cct etc ggg gee cga 1345 
Glu Gly His Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Are 
430 435 440 445 

gga ggg acc ctg gag ctg cgc ccg cga tct cgc tac cgt tta cag ctg 1393 
Gly Gly Thr Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu 
450 455 460 

cgc gec agg etc aac ggc ccc acc tac caa ggt ccc tgg age teg tgg 1441 
Arg Ala Arg Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp 
465 470 475 

teg gac cca act agg gtg gag acc gec acc gag acc gee tgg ate tec 1489 
Ser Asp Pro Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser 
480 485 490 

T ttg £*? 2P C gCt Ctg Cat Cta gtg ctg ggc etc age gec gtc ctg ggc 
Leu Val Thr Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly 
495 500 505 

ctg ctg ctg ctg agg tgg cag ttt cct gca cac tac agg aga ctg agg 
Leu Leu Leu Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg 
510 515 520 525 

cat gec ctg tgg ccc tea ctt cca gac ctg cac egg gtc cta ggc cag 
His Ala Leu Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin 
530 535 540 

tac ctt agg gac act gca gec ctg age ccg ccc aag gec aca gtc tea 
Tyr Leu Arg Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser 
545 550 555 

? at ?u° ^ gt £* P a gtg gaa ccc ^ ctc ctt gaa ate etc ccc aag 
Asp Thr Cys Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys 
560 565 570 



1537 



1585 



1633 



1681 



1729 



tec tea gag agg act cct ttg ccc ctg tgt tec tec cag gec cag atg 1777 

ffilE#2 005-3004069 



#M 2004-071763 

Ser Ser Glu Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met 
575 580 585 



^-S>: 98/ 



gac tac cga aga ttg cag cct tct tgc ctg ggg acc atg ccc ctg tct 1825 
Asp Tyr Arg Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser 
590 595 600 605 

gtg tgc cca ccc atg get gag tea ggg tec tgc tgt acc acc cac att 1873 
Val Cys Pro Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He 
610 615 620 



gec aac cat tec tac eta cca eta age tat tgg cag cag cct tga 
Ala Asn His Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 

gtcgac 



<210> 250 

<211> 635 

<212> PRT 

<213> Homo sapiens 

<400> 250 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu Leu Leu Ala 
1 5 10 15 

Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser Leu Leu Ala 
20 25 30 

Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe Glu Asp Leu 
35 40 45 

Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly Thr Tyr Gin 
50 55 60 

Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys Pro Leu Ser 
65 7 0 75 go 

Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr Val Cys Gin Phe Pro 
85 90 95 

Asp Gin Glu Glu Val Arg Leu Phe Phe Pro Leu His Leu Trp Val Lys 
100 105 no 

Asn Val Phe Leu Asn Gin Thr Arg Thr Gin Arg Val Leu Phe Val Asd 
H5 120 125 

Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala Met Glv Glv 
130 135 140 



1918 



1924 



&iE# 2005-3004069 



#M 2004-071763 

% 

Ser Gin Pro Gly Glu Leu Gin lie Ser Trp Glu Glu Pro Ala Pro Glu 

155 160 

He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly P ro Arg Asp Pro 

Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala Thr Glu Thr 
180 185 190 

Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala Leu Asp Gin 

JUV 205 

Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Cly Pro Lys Gin 

215 220 
Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu Gly Gly Ser 

235 240 
Cys Leu lie Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp Leu Gin Leu 

250 255 
Cys Ser Gin Pro Asp Gly He Ser Leu Gly Gly Ser Trp Gly Ser Trp 

J65 270 
Ser Leu Pro Val Thr Val Asp Leu Pro Gly Asp Ala Val Ala Leu Gly 

^° 285 

Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys Gin Trp Gin 

^ 300 
Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His Ser Arg Ala 

315 320 
Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn Cys Glu Glu 
Ui 330 335 

Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe Ser Arg Cys 

645 350 
His Phe Lys Ser Arg Asn Asp Ser He lie His He Leu Val Glu Val 



^-v. 99/ 



360 



365 



Thr Thr Ala Pro Gly Thr Val His Ser Tyr Uu Gly Ser Pro Phe Trp 

6(b 380 
He His Gin Ala Val Are Lph p™ ti,,- d a 

385 390 ° Thr Pro Asn Leu His Trp Arg Glu 

395 400 

He Ser Ser Gly His Leu G.u Uu Glu Trp Gin His Pro Ser Ser Trp 

tbfE# 2005-3004069 



2004-071763 



^-'J : 100/ 



405 



410 



415 



Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly Glu Gly His 
420 425 430 

Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg Gly Gly Thr 
435 440 445 

Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu Arg Ala Arg 
450 455 460 

Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp Ser Asp Pro 
465 470 475 480 

Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser Leu Val Thr 
485 490 495 

Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly Leu Leu Leu 
500 505 510 

Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg His Ala Leu 
515 520 525 

Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin Tyr Leu Arg 
530 535 540 

Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser Asp Thr Cys 
545 550 555 560 

Glu Glu Val Glu Pro Ser Leu Leu Glu lie Leu Pro Lys Ser Ser Glu 
565 570 575 

Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met Asp Tyr Arg 
580 585 590 

Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser Val Cys Pro 
595 600 605 

Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He Ala Asn His 
610 615 620 



Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 



<210> 251 

<211> 1924 

<212> DNA 

<213> Homo sapiens 



ffifE# 2005-3004069 



#® 2004 - 071763 

<220> 

<221> CDS 

<222> (11).. (1918) 

<223> 

<400> 251 

gaattccacc atg ccc tec tgg gee etc ttc atg gtc acc tec tgc etc 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu 
1 5 10 

etc ctg gee cct caa aac ctg gee caa gtc age age caa gat gtc tec 
Leu Leu Ala Pro Gin Asn Leu Ala Gin Val Ser Ser Gin £p Val Ser 
lb 20 



^-v: 101/ 



125 



III f C Q gt ?f f tg CCg gCt CCC CCC atc atc aa g ^c 
Phe Val Asp Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala 

130 135 240 

2*? ?f f? g o gC Cag CCa ggg gaa ctt c *8 at c age tgg gag sag cca 
Met Gly Gly Ser Gin Pro Gly Glu Leu Gin He sL T?p Su £5 Pro 
145 150 155 

get cca gaa atc agt gat ttc ctg agg tac gaa etc cgc tat ssc ccc 
Ala Pro Glu He Ser Asp Phe Leu Arg Tyr §i u LeS A?g £ Pro 
IDU 165 270 



49 



97 



25 

ttg ctg gca tea gac tea gag ccc ctg aag tgt ttc tec cga aca ttt 145 
Leu Leu Ala Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg ?hr Phe 

35 40 45 



gag gac etc act tgc ttc tgg gat gag gaa gag gca gcg ccc art sss 
Glu Asp Leu Thr Cys Phe Trp Asp Glu Glu Glu Ala flf Fro Ser G?y 
50 55 60 

tZ ^ C rf r Ctg T Ctg l at gCC taC ccg cgg gag aa ^ ccc cgt get tgc 
Thr Tyr Gin Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys 

65 70 75 

ccc ctg agt tec cag age atg ccc cac ttt gga acc cga tac gtg tgc 
Pro Leu Ser Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr faf Cys 
80 85 90 

S Ca ? 3C Cag gag gaa gtg cgt ctc ttc ttt ccg ctg cac etc 
Gin Phe Pro Asp Gin Glu Glu Val Arg Leu Phe Phe ?ro Lei Sis LeS 
95 100 105 

tgg gtg aag aat gtg ttc eta aac cag act egg act cag cga etc ctc W 
Trp Val Lys Asn Val Phe Leu Asn Gin Thr a£ Thr G Tn Ar g vll Leu 
A -W 115 120 



193 



241 



289 



337 



433 



481 



529 



tfiffiE# 2005-3004069 



#M 2004-071763 

aga gat ccc aag aac tec act ggt ccc acg gtc ata cag ctg att gec 
Arg Asp Pro Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala 
!75 180 185 



^-v: 102/ 

577 



aca gaa acc tgc tgc cct get ctg cag aga cct cac tea gee tct get 625 
Thr Glu Thr Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala 
190 195 200 205 

ctg gac cag tct cca tgt get cag ccc aca atg ccc tgg caa gat gga 673 
Leu Asp Gin Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly 
210 215 220 

cca aag cag acc tec cca agt aga gaa get tea get ctg aca gca gag 721 
Pro Lys Gin Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu 
225 230 235 

ggt gga age tgc etc ate tea gga etc cag cct ggc aac tec tac tgg 769 
Gly Gly Ser Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp 
240 245 250 

ctg cag ctg cgc age gaa cct gat ggg ate tec etc ggt ggc tec tgg 817 
Leu Gin Leu Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp 
255 260 265 

gga tec tgg tec etc act gtg act gtg gac ctg cct gga gat gca gtg 865 
Gly Ser Trp Ser Leu Thr Val Thr Val Asp Leu Pro Gly Asp Ala Val 
270 2 75 280 285 

gca ctt gga ctg caa tgc ttt acc ttg gac ctg aag aat gtt acc tgt 913 
Ala Leu Gly Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys 
290 295 300 

caa tgg cag caa cag gac cat get age tec caa ggc ttc ttc tac cac 961 
Gin Trp Gin Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His 
305 310 315 

age agg gca egg tgc tgc ccc aga gac agg tac ccc ate tgg gag aac 1009 
Ser Arg Ala Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn 
320 325 330 

tgc gaa gag gaa gag aaa aca aat cca gga eta cag acc cca cag ttc 1057 
Cys Glu Glu Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe 
335 340 345 

tct cgc tgc cac ttc aag tea cga aat gac age att att cac ate ctt 1105 
Ser Arg Cys His Phe Lys Ser Arg Asn Asp Ser lie He His He Leu 
350 355 360 365 

5*? ?f g 5*? 2* aca gcc ccg ggt act 8" cac a g c ctg ggc tec 1153 
Val Glu Val Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser 

ffliE# 2005-3004069 



#® 2004-071763 
370 375 



380 



p H C i 88 r\° ?? C Cag gCt gtg csc ctc ccc acc cca aac tte cac 
Pro Phe Trp le His Gin Ala Val Arg Leu Pro Thr Pro Asn llu ml 
385 390 395 

T? An? S5 ?E £° ?* rf Ctg ^ " g gag ^ cca 
Trp Arg Glu He Ser Ser Gly His Leu Glu Leu Glu Trp Gin His Pro 

^ uu 405 420 

teg tec tgg gca gee caa gag acc tgt tat caa ctc cga tac ara «« 
Ser Ser Trp Ala Ala Gin Glu Thr Cys Tyr Gin Leu A?g £ £ 
4Ib 42 0 425 

nl rf S at rf f C tgg aag gtg ctg «a« ccg cct ctc ggg gcc cga 
Glu Gly His Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Qy Tit A?g 

4J5 440 445 

ffv ffv ru° r Ctg f ag T Ctg f C CCg Cga tct <* c tac cgt tta cag ctg 
Gly Gly Thr Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu 

450 455 460 

cgc gcc agg ctc aac ggc ccc acc tac caa ggt ccc tgg aec w t(W 
Arg Ala Arg Leu Asn Gly Pro Thr Tyr Gin G?y Pro T? P Ser Se? rip 
465 470 475 

teg gac cca act agg gtg gag acc gcc acc gag acc gcc tee atr trr 
Ser Asp Pro Thr Arg Val Glu Thr Ala Thr ?lu ttr Tit T?p He Ser 
8U 485 490 

T^n f f TU C § f T Ctg Cta gtg Ctg SSC CtC ^C gCC gtC Ctg gg C 

Leu Val Thr Ala Leu His Leu Val Leu Gly Leu SeV 1 la fa? 2l cfy 
4yi? 500 505 

ctg ctg ctg ctg agg tgg cag ttt cct gca cac tac agg aga ctg zee 
Leu Leu Leu Leu Arg Trp Gin Phe Pro Ala His Tyr A?g A?g £u 1% 

515 520 525 

cat gcc ctg tgg ccc tea ctt cca gac ctg cac egg gtc cta mc cae 
His Ala Leu Trp Pro Ser Leu Pro Asp Leu His A?g &f Leu Gly Gin 
530 535 54 0 

tac ctt agg gac act gca gcc ctg age ccg ccc aag gcc aca gtc tea 
Tyr Leu Arg Asp Thr Ala Ala Leu Ser Pro Pro Ly? I la Thr vll Ser 
545 550 555 

fsp c£ Hn {? f S CC 880 CtC Ctt gaa atc ct ^ ccc aag 
Asp Thr Cys Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys 

560 565 570 



1201 



1249 



1297 



1345 



1393 



1441 



1489 



1537 



1585 



1633 



1681 



1729 



103/ 



tBffiE#2 005-3004069 



#M 2004-071763 



gcc aac cat tec tac eta cca eta age tat tgg cag cag cct tga 
Ala Asn His Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 

gtcgac 



I 104/ 



tec tea gag agg act cct ttg ccc ctg tgt tec tec cag gcc cag atg 1777 
Ser Ser Glu Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met 
575 580 585 

gac tac cga aga ttg cag cct tct tgc ctg ggg ace atg ccc ctg tct 1825 
Asp Tyr Arg Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser 
590 595 600 605 

gtg tgc cca ccc atg get gag tea ggg tec tgc tgt ace ace cac att 1873 
Val Cys Pro Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He 
610 615 620 



1918 



1924 



<210> 252 

<211> 635 

<212> PRT 

<213> Homo sapiens 

<400> 252 

Met Pro Ser Trp Ala Leu Phe Met Val Thr Ser Cys Leu Leu Leu Ala 
1 5 10 15 

Pro Gin Asn Leu Ala Gin Val Ser Ser Gin Asp Val Ser Leu Leu Ala 
20 25 30 

Ser Asp Ser Glu Pro Leu Lys Cys Phe Ser Arg Thr Phe Glu Asp Leu 
35 40 45 

Thr Cys Phe Trp Asp Glu Glu Glu Ala Ala Pro Ser Gly Thr Tyr Gin 
50 55 60 

Leu Leu Tyr Ala Tyr Pro Arg Glu Lys Pro Arg Ala Cys Pro Leu Ser 
65 70 75 80 

Ser Gin Ser Met Pro His Phe Gly Thr Arg Tyr Val Cys Gin Phe Pro 
85 90 95 

Asp Gin Glu Glu Val Arg Leu Phe Phe Pro Leu His Leu Trp Val Lys 
100 105 no 

Asn Val Phe Leu Asn Gin Thr Arg Thr Gin Arg Val Leu Phe Val Asp 
115 120 125 

Ser Val Gly Leu Pro Ala Pro Pro Ser He He Lys Ala Met Gly Gly 

mtE# 2005-3004069 



#M 2004-071763 



^-v: 105/ 



130 



135 



140 



Ser Gin Pro Gly Glu Leu Gin He Ser Trp Glu Glu Pro Ala Pro Glu 
145 150 155 160 

He Ser Asp Phe Leu Arg Tyr Glu Leu Arg Tyr Gly Pro Arg Asp Pro 
165 170 175 

Lys Asn Ser Thr Gly Pro Thr Val He Gin Leu He Ala Thr Glu Thr 
180 185 190 

Cys Cys Pro Ala Leu Gin Arg Pro His Ser Ala Ser Ala Leu Asp Gin 
195 200 205 

Ser Pro Cys Ala Gin Pro Thr Met Pro Trp Gin Asp Gly Pro Lys Gin 
210 215 220 

Thr Ser Pro Ser Arg Glu Ala Ser Ala Leu Thr Ala Glu Gly Gly Ser 
225 230 235 ' 240 

Cys Leu He Ser Gly Leu Gin Pro Gly Asn Ser Tyr Trp Leu Gin Leu 
245 250 255 

Arg Ser Glu Pro Asp Gly He Ser Leu Gly Gly Ser Trp Gly Ser Trp 
260 265 270 

Ser Leu Thr Val Thr Val Asp Leu Pro Gly Asp Ala Val Ala Leu Gly 
275 280 285 

Leu Gin Cys Phe Thr Leu Asp Leu Lys Asn Val Thr Cys Gin Trp Gin 
290 295 300 

Gin Gin Asp His Ala Ser Ser Gin Gly Phe Phe Tyr His Ser Arg Ala 
305 310 315 320 

Arg Cys Cys Pro Arg Asp Arg Tyr Pro He Trp Glu Asn Cys Glu Glu 
325 330 335 

Glu Glu Lys Thr Asn Pro Gly Leu Gin Thr Pro Gin Phe Ser Arg Cys 
340 345 350 

His Phe Lys Ser Arg Asn Asp Ser He He His He Leu Val Glu Val 
355 360 365 

Thr Thr Ala Pro Gly Thr Val His Ser Tyr Leu Gly Ser Pro Phe Trp 
370 375 380 

He His Gin Ala Val Arg Leu Pro Thr Pro Asn Leu His Trp Arg Glu 



385 



390 



395 



400 



tBIDMf 2005-3004069 



#M 2004-071763 

He Ser Ser Gly His Leu Glu Leu Glu Tip Gin His Pro Ser Ser Trp 
405 410 415 

Ala Ala Gin Glu Thr Cys Tyr Gin Leu Arg Tyr Thr Gly Glu Gly His 



-^-v: 106/ 



420 



425 



430 



Gin Asp Trp Lys Val Leu Glu Pro Pro Leu Gly Ala Arg Gly Gly Thr 
435 440 445 

Leu Glu Leu Arg Pro Arg Ser Arg Tyr Arg Leu Gin Leu Arg Ala Arg 
450 455 460 

Leu Asn Gly Pro Thr Tyr Gin Gly Pro Trp Ser Ser Trp Ser Asp Pro 
465 470 475 480 

Thr Arg Val Glu Thr Ala Thr Glu Thr Ala Trp He Ser Leu Val Thr 
485 490 495 

Ala Leu His Leu Val Leu Gly Leu Ser Ala Val Leu Gly Leu Leu Leu 
500 505 510 

Leu Arg Trp Gin Phe Pro Ala His Tyr Arg Arg Leu Arg His Ala Leu 
515 520 525 

Trp Pro Ser Leu Pro Asp Leu His Arg Val Leu Gly Gin Tyr Leu Arg 
530 535 540 

Asp Thr Ala Ala Leu Ser Pro Pro Lys Ala Thr Val Ser Asp Thr Cvs 
545 550 555 560 

Glu Glu Val Glu Pro Ser Leu Leu Glu He Leu Pro Lys Ser Ser Glu 
565 570 575 

Arg Thr Pro Leu Pro Leu Cys Ser Ser Gin Ala Gin Met Asp Tyr Arg 
580 585 590 

Arg Leu Gin Pro Ser Cys Leu Gly Thr Met Pro Leu Ser Val Cys Pro 
595 600 605 

Pro Met Ala Glu Ser Gly Ser Cys Cys Thr Thr His He Ala Asn His 
610 615 620 

Ser Tyr Leu Pro Leu Ser Tyr Trp Gin Gin Pro 
625 630 635 



<210> 253 

<211> 1572 

<212> DNA 

<213> Homo sapiens 



mEE# 2005-3004069 



#H 2004-071763 



^-v : 107/ 



<400> 253 

atggactgga cctggaggtt cctctttgtg gtggcagcag ctacaggtgt ccagtcccag 60 

gtgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 120 

tgcaaggctt ctggatacac cttcaccaac tcctggatga actgggtgag gcagaggcct 180 

ggaaagggtc ttgagtgggt tggacggatt tatcctggag atggagaaac tatctacaat 240 

gggaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 300 

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 360 

gattactcgt ttgcttactg gggccaggga accacggtca ccgtctcttc aggtggtggt 420 

ggatccggag' gtggtggatc gggtggtgga ggatcggata ttgtgatgac tcagtctgca 480 

ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 540 

ctcctgcata gtaatggcaa cacttacttg tattggtacc tgcagaagcc agggcagtct 600 

ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 660 

ggcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 720 

ggggtttatt actgcatgca acatatagaa tatcctttta cgttcggcca agggaccaaa 780 

ctggaaatca aaggaggtgg tggatcgggt ggtggtggtt cgggaggcgg tggatcgcag 840 

gtgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 900 

tgcaaggctt ctggatacac cttcaccaac tcctggatga actgggtgag gcagaggcct 960 

ggaaagggtc ttgagtgggt tggacggatt tatcctggag atggagaaac tatctacaat 1020 

gggaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 1080 

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 1140 

gattactcgt ttgcttactg gggccaggga accacggtca ccgtctcttc aggtggtggt 1200 

ggatccggag gtggtggatc gggtggtgga ggatcggata ttgtgatgac tcagtctgca 1260 

ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 1320 

ctcctgcata gtaatggcaa cacttacttg tattggtacc tgcagaagcc agggcagtct 1380 

ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 1440 

ttSfE# 2005-3004069 



#® 2004-071763 v • 

ggcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 1500 
ggggtttatt actgcatgca acatatagaa tatcctttta cgttcggcca agggaccaaa 1560 
ctggaaatca aa 

ID/ 6 

<210> 254 
<211> 524 
<212> PRT 
<213> Homo sapiens 

<400> 254 

Met Asp Trp Thr Trp Arg Phe Leu Phe Val Val Ala Ala Ala Thr Gly 
15 10 15 

Val Gin Ser Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys 
20 25 30 

Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
35 40 45 

Thr Asn Ser Trp Met Asn Trp Val Arg Gin Arg Pro Gly Lys Gly Leu 
50 55 g0 

Glu Trp Val Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 
65 70 75 go 

Gly Lys Phe Arg Val Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser 
85 90 95 

Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
100 105 no 

Tyr Tyr Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Glv 
115 120 125 

Gin Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
130 135 14Q 

Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met Thr Gin Ser Ala 
145 150 155 160 

Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser He Ser Cys Arg 
165 170 175 

Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr Trn 
180 185 190 

Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu He Tyr Arg Met 

&SE^2 005-3004069 



2004-071763 



^-v: 109/ 



195 



200 



205 



Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
210 215 220 

Gly Thr Ala Phe Thr Leu Lys He Ser Arg Val Glu Ala Glu Asp Val 
225 230 235 240 

Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro Phe Thr Phe Gly 
245 250 255 

Gin Gly Thr Lys Leu Glu He Lys Gly Gly Gly Gly Ser Gly Gly Gly 
260 265 270 

Gly Ser Gly Gly Gly Gly Ser Gin Val Gin Leu Val Gin Ser Gly Pro 
275 280 285 

Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
290 295 300 

Gly Tyr Thr Phe Thr Asn Ser Trp Met Asn Trp Val Arg Gin Arg Pro 



310 



315 



320 



Gly Lys Gly Leu Glu Trp Val Gly Arg He Tyr Pro Gly Asp Gly Glu 
325 330 335 

Thr He Tyr Asn Gly Lys Phe Arg Val Arg Val Thr He Thr Ala Asp 
340 345 350 

Glu Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu 
355 360 365 

Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe 
370 375 380 

385 ?yr Trp Gly Gln oil Thr Thr Val ^ Val Ser Ser G1 y G1 y Gly 



390 



395 



400 



Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met 
405 410 415 

Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser 
420 425 430 

He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr 
435 440 445 

Tyr Leu Tyr Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu 
450 455 460 



ftiE#2 005-3004069 



#JM 2004 - 071763 ^- *J : no/ 

He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
465 470 475 * 480 

Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Lys He Ser Arg Val Glu 
485 490 495 

Ala Glu Asp. Val Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro 
500 505 510 

Phe Thr Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
515 520 



<210> 255 

<211> 354 

<212> DNA 

<213> Homo sapiens 

<400> 255 

caggtgcagc tggtgcagtc tggacctgag gtgaagaagc ctggggcctc agtgaaggtc 60 

tcctgcaagg cttctggata caccttcacc aactcctgga tgaactgggt gaggcagagg 120 

cctggaaagg gtcttgagtg ggttggacgg atttatcctg gagatggaga aactatctac 180 

aatgggaaat tcagggtcag agtcacgatt accgcggacg aatccacgag cacagcctac 240 

atggagctga gcagcctgag atctgaggac acggccgtgt attactgtgc gagaggctat 300 

gatgattact cgtttgctta ctggggccag ggaaccacgg tcaccgtctc ttca 354 

<210> 256 

<211> 118 

<212> PRT 

<213> Homo sapiens 

<400> 256 

Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Ser 
20 25 30 

Trp Met Asn Trp Val Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp Val 
35 40 45 

Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe 
50 55 60 

ffitE#2 005-30040 69 



12 0 0 4-0 7 1 7 6 3 ^-v? : 111/ 

Arg Val Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65 70 75 go 

Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
85 90 95 

Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
!00 105 no 

Thr Val Thr Val Ser Ser 
115 



<210> 257 

<211> 336 

<212> DNA 

<213> Homo sapiens 



<210> 258 

<211> 112 

<212> PRT 

<213> Homo sapiens 



<400> 258 

Asp He Val Met Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Glv 
1 5 10 - 



15 



Glu Pro Ala Ser He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
20 25 30 

Asn Gly Asn Thr Tyr Leu Tyr Trp Tyr Leu Gin Lys Pro Gly Gin Ser 
35 40 45 

Pro Gin Leu Leu He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
50 55 60 



60 



<400> 257 

gatattgtga tgactcagtc tgcactctcc ctgcccgtca cccctggaga gccggcctcc 
atctcctgca ggtctagtaa gagtctcctg catagtaatg gcaacactta cttgtattgg 120 
tacctgcaga agccagggca gtctccacag ctcctgatct atcggatgtc caaccttgcc 



180 



tcaggggtcc ctgacaggtt cagtggcagt ggatcaggca cagcttttac actgaaaatc 240 
agcagagtgg aggctgagga tgttggggtt tattactgca tgcaacatat agaatatcct 
tttacgttcg gccaagggac caaactggaa atcaaa 



300 
336 



tBIE#2 005-3004069 



9 



#M 2004-071763 

Asp Arg P he Ser Gly Ser G, y Ser Gly Thr Ala Phe Thr Leu Lys He 

75 80 
Ser Arg Val Glu Ala GIu Asp Val Gly Val Tyr Tyr Cys Met Gin His 



90 



95 



He Glu Tyr Pro Phe Thr Phe Gly Gin Gly Thr Lys Leu Glu He Lys 

105 11Q 



<210> 259 

<211> 1572 

<212> DNA 

<213> Homo sapiens 

<400> 259 

atggactgga cctggaggtt cctctttgtg gtggcagcag ctacaggtgt ccagtcccag 
Stgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 
tgcaaggctt ctggatacac cttcaccaac tcctggatga actggatcag gcagaggcct 
ggaaagggtc ttgagtggat tggacggatt tatcctggag atggagaaac tatctacaat 
gggaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 
gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 
gattactcgt ttgcttactg gggccaggga accctggtca ccgtctcttc aggtggtggt 
ffiatccggag gtggtggatc gggtggtgga ggatcggata ttgtgatgac tcagtctgca 
ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 
ctcctgcata gtaatggcaa cacttacttg tattggtacc tgcagaagcc agggcagtct 
ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 
Kcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 
BBttUtt actgcatgca acatatagaa tatcctttta cgttcggcca agggaccaaa 
ctggaaatca aaggaggtgg tggatcgggt ggtggtggtt cgggaggcgg tggatcgcag 
Stgcagctgg tgcagtctgg acctgaggtg aagaagcctg gggcctcagt gaaggtctcc 
tgcaaggctt ctggatacac cttcaccaac tcctggatga actggatcag gcagaggcct 
ggaaagggtc ttgagtggat tggacggatt tatcctggag atggagaaac tatctacaat 



60 
120 
180 
240 
300 
360 
420 
480 
540 
600 
660 
720 
780 
840 
900 
960 
1020 

ffilE#2 005-300406 



9 



'2 004-071763 



113/ 



Kgaaattca gggtcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg !080 

gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgag aggctatgat 1140 

gattactcgt ttgcttactg gg gccaggga accctggtca ^ 

ffiatccggag gtggtggatc gggtggtgga ggatcggata ^ 

ctctccctgc ccgtcacccc tggagagccg gcctccatct cctgcaggtc tagtaagagt 1320 
ctcctgcata gtaat gg caa cacttacttg tatt gg tacc tgcagaagcc agggcagtct 1380 
ccacagctcc tgatctatcg gatgtccaac cttgcctcag gggtccctga caggttcagt 1440 
Egcagtggat caggcacagc ttttacactg aaaatcagca gagtggaggc tgaggatgtt 1500 
«*tt.tt actgcatgca acatatagaa tatcctttta cg.tcggcca a ggg accaaa 1560 
ctggaaatca aa 

1572 

<210> 260 
<211> 524 
<212> PRT 
<213> Homo sapiens 

<400> 260 

Met Asp Trp Thr Trp Ar g Phe Leu Phe Val Val A,a Ala Ala Thr Gly 

10 15 

i Ser Gly Pro Glu Val 
25 30 

' Cys Lys Ala Ser Gly 
4 0 45 

) He Arg Gin Arg Pro 
55 60 

j 

75 80 

r Ala Asp Glu Ser Thr 
9 0 95 

Thr Ala Tyr Met Glu Leu Ser Ser Lph a™ c n, a m 

100 7^ g Ser Glu As P ^ A1 * Val 

105 I10 

Tyr Tyr Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly 

izu 125 

ffi§E#2 005-3004069 



Val Gin Ser Gin Val Gin Leu Val Gin Ser G.y Pro Glu Va, Lys Lys 

25 30 

Pro Gly Ala Ser Val Lys Va. Ser Cys Lys Ala Ser Gly Tyr Thr Phe 

40 45 

Thr Asn Ser Trp Met Asn Trp He Arg Gin Arg P r0 Gly Lys Gly Leu 

65 60 
Glu Trp He Gly Ar g lie Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 

75 80 
Gly Lys Phe Ar g Val Arg Va, Thr He Thr Ala Asp Glu Ser Thr Ser 



#® 2004-071763 
S ^ ^ ™ S - ^ «> Cly Gly G iy Ser G, y G, y 

135 140 * ui y 

Giy G,y Ser Gly G, y G,y G,y Ser Asp He Va, Met Thr G,„ Se r Ala 

155 16Q 
Leu Se r Leu Pro Va, Thr P r0 Gly Glu ^ ^ ^ ^ 

I/u 175 
Ser Ser Lys Ser Leu Leu His Se r Asn G.y As„ Thr Tyr Uu Tyr Trp 

185 190 
Tyr Leu Gin Lys P r „ Gly Gln S Pro Gln Leu Leu ^ ^ 

^ UU 205 
Ser Asn Leu Ala Ser Gly Va, Pro Asp Ar g Phe Ser Gly Ser Gly Ser 

6it> 220 
Gly Thr Ala Phe Thr Leu Lys He Ser Ar g Va, Glu Ala Glu Asp Va, 

235 240 
Giy Va, Tyr Tyr ^ Met Gln His ^ ^ ^ ^ ^ ^ 

255 

Gin Gly Thr ^ Leu Glu He Lys G.y G,y Gly GIy Ser CJy G , y 

^bb 27 Q 

Giy Ser G, y G.y Gly Gly Ser Gln Va, Gln Leu Va. G.n Ser G.y P r0 

zsu 285 
Glu Va, Lys Lys P r0 G,y Al a Ser Va, Lys Va. Ser Cys Lys A.a Ser 

G.y Tyr Thr Phe Thr Asn Ser Trp Met Asn Trp He Ar g Gln Ar g Pro 

315 320 
Giv Lys Gly Leu Glu Trp He Gly Ar g He Tyr Pro Gly Asp G.y Glu 

335 

Thr He Tyr Asn Gly Lys Phe Ar g Va, Ar g Va, Thr He Thr A,a Asp 

345 350 
Glu Ser £ Ser Thr A,a Tyr Met Glu Leu Ser Ser Leu Ar g Ser Glu 

aw 365 
Asp Thr Ala Va, Tyr Tyr Gys Ala Ar g Gly Tyr Asp Asp Tyr Ser Phe 



375 380 



114/ 



Ala Tyr Trp G,y G , n GIy ^ ^ Va] ^ ftj ^ ^ ^ ^ ^ 

tt}|E#2 005-3004069 



#®2 0 0 4 - 0 7 1 7 6 3 
385 390 395 400 

Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met 
405 410 415 

Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser 
420 425 430 

He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr 
435 440 445 

Tyr Leu Tyr Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu 
450 455 460 

He Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
465 470 475 480 

Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Lys He Ser Arg Val Glu 
485 490 495 

Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro 
500 505 510 

Phe Thr Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
515 520 



<210> 261 

<211> 354 

<212> DNA 

<213> Homo sapiens 

<400> 261 

caggtgcagc tggtgcagtc tggacctgag gtgaagaagc ctggggcctc agtgaaggtc 60 

tcctgcaagg cttctggata caccttcacc aactcctgga tgaactggat caggcagagg 120 

cctggaaagg gtcttgagtg gattggacgg atttatcctg gagatggaga aactatctac 180 

aatgggaaat tcagggtcag agtcacgatt accgcggacg aatccacgag cacagcctac 240 

atggagctga gcagcctgag atctgaggac acggccgtgt attactgtgc gagaggctat 300 

gatgattact cgtttgctta ctggggccag ggaaccctgg tcaccgtctc ttca 354 

<210> 262 

<211> 118 

<212> PRT 

<213> Homo sapiens 



115/ 



miiE# 2 0 0 5-3 0 



0 4 0 6 9 



2004-071763 



^-v: 116/ 



<400> 262 



Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Ser 
20 25 30 

Trp Met Asn Trp He Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp He 
35 40 45 

Gly Arg lie Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe 
50 55 60 

Arg Val Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65 70 75 80 

Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
85 90 95 

.Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly Gin Gly Thr 
!00 105 no 

Leu Val Thr Val Ser Ser 
115 



<210> 263 

<211> 1572 

<212> DNA 

<213> Mus musculus 

<400> 263 

atggaatggc ctttgatctt tctcttcctc ctgtcaggaa ctgcaggtgt ccactcccag 60 

gttcagctgc agcagtctgg acctgagctg gtgaagcctg gggcctcagt gaagatttcc 120 

tgcaaggctt ctggctatgc attcactaac tcctggatga actgggtgaa gcagaggcct 180 

ggaaagggtc ttgagtggat tggacggatt tatcctggag atggagaaac tatctacaat 240 

gggaaattca gggtcaaggc cacactgact gcagacaaat cctccagcac agcctacatg 300 

gatatcagca gcctgacatc tgaggactct gcggtctact tctgtgcaag aggctatgat 360 

gattactcgt ttgcttactg gggccaaggg actctggtca ctgtctctgc aggtggtggt 420 

ggttcgggtg gtggtggttc gggtggtggc ggatcggata ttgtgatgac tcaggctgca 480 

ccctctatac ctgtcactcc tggagagtca gtatccatct cctgtaggtc tagtaagagt 540 

tfJSE# 2005-3004069 



#12 0 0 4 -0 7 1 7 6 3 ^-v : 117/ 

ctcctgcata gtaatggcaa cacttacttg tattggttcc tgcagaggcc aggccagtct 600 

cctcaactcc tgatatatcg gatgtccaac cttgcctcag gagtcccaga taggttcagt 660 

ggcagtgggt caggaactgc tttcacactg agaatcagta gagtggaggc tgaggatgtg 720 

ggtgtttatt actgtatgca acatatagaa tatcctttta cgttcggatc ggggaccaag 780 

ctggaaataa aaggaggtgg tggatcgggt ggtggtggtt cgggaggcgg tggatcgcag 840 

gttcagctgc agcagtctgg acctgagctg gtgaagcctg gggcctcagt gaagatttcc 900 

tgcaaggctt ctggctatgc attcactaac tcctggatga actgggtgaa gcagaggcct 960 

ggaaagggtc ttgagtggat tggacggatt tatcctggag atggagaaac tatctacaat 1020 

gggaaattca gggtcaaggc cacactgact gcagacaaat cctccagcac agcctacatg 1080 

gatatcagca gcctgacatc tgaggactct gcggtctact tctgtgcaag aggctatgat 1140 

gattactcgt ttgcttactg gggccaaggg actctggtca ctgtctctgc aggtggtggt 1200 

ggttcgggtg gtggtggttc gggtggtggc ggatcggata ttgtgatgac tcaggctgca 1260 

ccctctatac ctgtcactcc tggagagtca gtatccatct cctgtaggtc tagtaagagt 1320 

ctcctgcata gtaatggcaa cacttacttg tattggttcc tgcagaggcc aggccagtct 1380 

cctcaactcc tgatatatcg gatgtccaac cttgcctcag gagtcccaga taggttcagt 1440 

ggcagtgggt caggaactgc tttcacactg agaatcagta gagtggaggc tgaggatgtg 1500 

ggtgtttatt actgtatgca acatatagaa tatcctttta cgttcggatc ggggaccaag 1560 
ctggaaataa aa 



1572 



<210> 264 

<211> 524 

<212> PRT 

<213> Mus musculus 

<400> 264 

Met GIu Trp Pro Leu He Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1 5 10 15 

Val His Ser Gin Val Gin Leu Gin Gin Ser Gly Pro Glu Leu Val Lys 
20 25 30 



ttiSEiff 2 005-3004069 



2004-071763 : 118/ 

Pro Gly Ala Ser Val Lys He Ser Cys Lys Ala Ser Gly Tyr Ala Phe 
35 40 45 

Thr Asn Ser Trp Met Asn Trp Val Lys Gin Arg Pro Gly Lys Gly Leu 
50 55 60 

Glu Trp He Gly Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn 
65 70 75 80 

Gly Lys Phe Arg Val Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
85 , 90 95 

Thr Ala Tyr Met Asp He Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
100 105 110 

Tyr Phe Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr Trp Gly 
115 120 125 

Gin Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly 
130 135 140 

Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met Thr Gin Ala Ala 
145 150 155 160 

Pro Ser He Pro Val Thr Pro Gly Glu Ser Val Ser He Ser Cys Arg 
165 170 175 

Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp 
180 185 190 

Phe Leu Gin Arg Pro Gly Gin Ser Pro Gin Leu Leu He Tyr Arg Met 
195 200 205 

Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
210 215 220 

Gly Thr Ala Phe Thr Leu Arg He Ser Arg Val Glu Ala Glu Asp Val 
225 230 235 240 

Gly Val Tyr Tyr Cys Met Gin His He Glu Tyr Pro Phe Thr Phe Gly 
245 250 255 

Ser Gly Thr Lys Leu Glu He Lys Gly Gly Gly Gly Ser Gly Gly Gly 
260 265 270 

Gly Ser Gly Gly Gly Gly Ser Gin Val Gin Leu Gin Gin Ser Gly Pro 
275 280 285 

Glu Leu Val Lys 'Pro Gly Ala Ser Val Lys He Ser Cys Lys Ala Ser 
290 295 300 

ffifiE# 2005-3004069 



#M 2004-071763 

w 

Gly Tyr Ala Phe Thr Asn Ser Trp Met Asn Trp Val Lys Gin Arg Pro 
5 310 315 320 

Gly Lys Gly Leu Glu Trp He Gly Arg He Tyr Pro Gly Asp Gly Glu 
325 330 335 

Thr He Tyr Asn Gly Lys Phe Arg Val Lys Ala Thr Leu Thr Ala Asp 
340 345 350 

Lys Ser Ser Ser Thr Ala Tyr Met Asp He Ser Ser Leu Thr Ser Glu 
Jbt) 360 365 

Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Tyr Asp Asp Tyr Ser Phe 
J ' u 375 380 

Ala Tyr Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly 

390 395 400 

Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp He Val Met 
405 410 415 

Thr Gin Ala Ala Pro Ser He Pro Val Thr Pro Gly Glu Ser Val Ser 
420 425 430 

He Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr 

440 445 

Tyr Leu Tyr Trp Phe Leu Gin Arg Pro Gly Gin Ser Pro Gin Leu Leu 
* ou 455 460 

lie Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 

/U 475 480 

Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg He Ser Arg Val Glu 



v : 119/ 



485 490 



495 



Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gin His lie Glu Tyr Pro 
500 505 510 

Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu He Lys 
515 520 



<210> 265 

<211> 30 

<212> PRT 

<213> Homo sapiens 

<400> 265 



£BfE#2 005-3004069 



m 



#H 2004-071763 

Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
20 25 30 



120/ 



<210> 266 

<211> 5 

<212> PRT 

<213> Homo sapiens 

<400> 266 

Asn Ser Trp Met Asn 

1 5 



<210> 267 

<211> 14 

<212> PRT 

<213> Homo sapiens 

<400> 267 

Trp Val Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp Val Gly 
1 5 10 



<210> 


268 


<211> 


17 


<212> 


PRT 


<213> 


Homo sapiens 


<400> 


268 



Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe Arg 
15 10 15 



Val 



<210> 269 

<211> 32 . 

<212> PRT 

<213> Homo sapiens 

<400> 269 

Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr Met Glu 
1 5 10 15 

Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 

ffi|E#2 005-3004069 



20 



2004-071763 

25 30 



s*-zs : 121/ 



<210> 270 

<211> 9 

<212> PRT 

<213> Homo sapiens 

<400> 270 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 


271 


<211> 


11 


<212> 


PRT 


<213> 


Homo 


<400> 


271 



Trp Gly Gin Gly Thr Thr Val Thr Val Ser Ser 
1 5 io 



<210> 272 

<211> 23 

<212> PRT 

<213> Homo sapiens 

<400> 272 

Asp He Val Met Thr Gin Ser Ala Leu Ser Leu Pro Val Thr Pro Gly 
15 10 15 

Glu Pro Ala Ser He Ser Cys 
20 



<210> 273 

<211> 16 

<212> PRT 

<213> Homo sapiens 



<400> 273 

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1 5 10 15 



<210> 274 

<211> 15 

<212> PRT 

<213> Homo sapiens 



ffifflE# 2005-3004069 



#M 2004-071763 



^-v: 122/ 



<400> 274 

Trp Tyr Leu Gin Lys Pro Gly Gin Ser Pro Gin Leu Leu He Tyr 
1 5 10 15 



<210> 275 

<211> 7 

<212> PRT 

<213> Homo sapiens 



<400> 275 

Arg Met Ser Asn Leu Ala Ser 
1 5 



<210> 


276 


<211> 


32 


<212> 


PRT 


<213> 


Homo 



<400> 276 

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr 
1 5 10 15 

Leu Lys He Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys 
20 25 30 



<210> 277 

<211> 9 

<212> PRT 

<213> Homo sapiens 

<400> 277 

Met Gin His He Glu Tyr Pro Phe Thr 
1 5 



<210> 


278 


<211> 


10 


<212> 


PRT 


<213> 


Homo 


<400> 


278 



Phe Gly Gin Gly Thr Lys Leu Glu He Lys 
1 5 10 



<210> 279 



ffifE# 2005-3004069 



2004-071 763 



123/ 



<211> 30 

<212> PRT 

<213> Homo sapiens 

<400> 279 

Gin Val Gin Leu Val Gin Ser Gly Pro Glu Val Lys Lys Pro Gly Ala 
1 5 10 15 

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
20 25 30 



<210> 280 

<211> 5 

<212> PRT 

<213> Homo sapiens 

<400> 280 

Asn Ser Trp Met Asn 

1 5 



<210> 


281 


<211> 


14 


<212> 


PRT 


<213> 


Homo 


<400> 


281 



Trp He Arg Gin Arg Pro Gly Lys Gly Leu Glu Trp He Gly 
1 5 10 



<210> 282 

<211> 17 

<212> PRT 

<213> Homo sapiens 

<400> 282 

Arg He Tyr Pro Gly Asp Gly Glu Thr He Tyr Asn Gly Lys Phe Arg 
1 5 10 * 15 

Val 



<210> 283 

<211> 32 

<212> PRT 

<213> Homo sapiens 



tiiiE# 2005-3004069 



#M 2004-071763 



^-v: 124/E 



<400> 283 

Arg Val Thr He Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr Met Glu 
15 10 15 

Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr fyr Cys Ala Arg 
20 25 30 



<210> 284 

<211> 9 

<212> PRT 

<213> Homo sapiens 

<400> 284 

Gly Tyr Asp Asp Tyr Ser Phe Ala Tyr 
1 5 



<210> 285 

<211> 11 

<212> PRT 

<213> Homo sapiens 

<400> 285 

Trp Gly Gin Gly Thr Leu Val Thr Val Ser Ser 
1 5 10 



aitiE#2 005-3004069 



[HI] 



#M 2004-071763 



1/ 



dbVB22B gi^jSMiili 



VL 



U>A— (Gly.Ser) 

-« 8 



-« 1— O 



PvuU 



1 



It 






VL 


n 



■J>*-(GI/ 4 Sei) 3 

PCRiSfa 



R-AGEH 



VB22B 



SCkrw^ vy^-A-Ejij 




1 



VL 



1 



Pvu ngiticscDira— r.>y 
Pvu n 



VL 




[EI 2 I 

CHO-human Mp l 




CHO-monkev Mpl 



VL 



FLAGB259 




i i i 1 1 



| < i ■ miiij I .i ■ 1 1 mi i ii,i mi 

xo* io 1 io" io» 



FITC LOG 



CHO-mouse Mpl 




1 I I I 1 1 M| I I ■ I 1 1 Hi I I I 1 1 1 H| I I t 1 ■ nil 
10° in» in 3 in» 



1Q 1 



FITC LOG 



io 1 



CHO 









Ml 

























i tiii in| ' i t 1 1 1 1 k| ; i 1 1 mil 
in* mi ir 



10* ID 1 lb 1 

FITC LOG 



1 1 1 mi 

10* 



fiiSE# 2005-3004069 



#M 2004-071763 



[0 3] 
1.6 




0.000 0.001 0.010 0.100 1.000 10.000 




0.000 0.001 0.010 0.100 1.000 10.000 

;tJt [nM] 



ffiSE# 2005-3004069 



Wm 2004-071763 



3/ 




0.0001 0.001 

[me] 



0.01 



0.1 



10 



:(nM) 



CDR1 



100 



CDR2 



VA7 

VA130 

VA259 

VB17B 

VB12B 

VB140 

VB33 

VB45B 

VB8B 

VB115 

VB14B 

VB22B 

VB16 

VB157 

VB4B 

VB51 



VA7 

VA130 

VA259 

VB17B 

VB12B 

VB140 

VB33 

VB45B 

VB8B 

VB115 

VB14B 

VB22B 

VB16 

VB157 

VB4B 

VB51 



QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQS G PELVKPGAS VKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCKASGYTFS 
QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCRAFGYAFS 
QVQLQQPGAELVKPGASVKLSCKASGYTFT 
QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCKASGYAFS 
QVQLQQSGPELLNPGAS VKI SCKASGYAFS 
QVQLQQSGPELVKPGASVKI SCKASGYAFT 
QVQLQQPGTELVRPGASVKLSCKASGYTFT 
QVQLQQPGAELVKPGASVKLSCKASGYT FT 
QVQLQQS GPELVKPGAS VKI S CKAS GYAFT 
QVQLQQS GPELVKPGAS VKI SCKAS GYAFS 



SSWMN 
SSWMN 
SSWMN 
SSWMN 
RSWMN 
NSWMN 
NYWVN 
SSWMN 
TSWMN 
SSWMN 
RSWMN 
NSWMN 
DYWVN 
DYWMN 
NSWMN 
NSWMN 



WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGRGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGPEWIG 
WVKQRPGKGLEWIG 
WVKQRPGKGLEWIG 
WVKQRPGRGLEWIG 
WVKQRPGRGLEWIG 
WVRQRPGKGLEWIG 
WVNQRPGKGLEWIG 

CDR3 



RTY PGDGDTNYNGKFKG 
RI Y PGDGDTNYNGKFKG 
RI Y PGDGETNYNGKFKG 
RIY PGDGDTNYNGKFKG 
RIYPGDGDTNYNGKFKG 
RIYPGDGETNNNGKFKG 
RIHPSDSETHCNQKFKR 
RIY PGDGETNNNGKFKG 
RI Y PGDGEANYNGKFKG 
RIYPGDGETNYNGKFKG 
RIY PGDGETNYNGKFKG 
RIY PGDGETI YNGKFRV 
RIHPYDSETHYNQKFKN 
RIHPFDSETHCSQKFKN 
RIY PGDGETI YNGKFRV 
RI Y PGDGDTI YNGNFKG 



KATLTADKS S S TAYMQLS S LT SEDS AVY FCAR 
KATLTADKSSSTAYIQLSSLTSEDSAVYFCAR 
KATLTADKS SNTAYMQLS S LT SE D S AVY FCAR 
KATLTADKSSSTAYMQLSSLTSEDSAVYFCAS 
KATLTADKSSSTAYMQLSSLTSEDSAVYFCAS 
KATLTADKS S S TAYMQLS S LT SE DS AVY FCAR 
KATLTVNKSSSTAYIQLHSLTSEDSAVYYCTS 
KATLTADKS S TT AYMQLS S LT SE DS AVY FCAR 
KATLTADKSSSSAYMQLSSLTSEDSAVYFCAR 
KATLTADKS S S T VYMQLS S LT SE D S AVY FCAR 
KATLTADKSSTTAYMQFSSLTSEDSAVYFCAR 
KATLTADKSS S TAYMDI S SLT S E D S AVY FCAR 
KATLT VDKS S STAY I QLS SLT SEDSAVY YCAS 
KATLTVDKSSNTAY IQFSSLT SEDSAVYYCS S 
KATLTADKSSSTAYMEISSLTSEDSAVYFCAR 
KATLTADKSS S I AYMQLS SLT SEDSAVY FCTS 



GWILADGGYSFAY 


GYAD- 


YSFAY 


GFGD- 


YSFAY 


GYAD- 


YSFAY 


GYDD- 


YSFAY 


GYGD- 


YSFAY 


GGW — 


FAY 


GYGD- 


YSFAY 


GYGD- 


YSFAY 


GYGD- 


YSFAY 


GDGD- 


YSFAY 


GYDD- 


YSFAY 








FAY 


GYDD- 


YSFAY 


GYDD- 


YSFAY 



WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 
WGQGTLVTVSA 



thiE#2 005-3004069 



#M 2004-071763 



^-y: 4/ 



[17] 



VA7 

VA130 

VA259 

VB17B 

VB12B 

VB140 

VB33 

VB45B 

VB8B 

VB115 

VB14B 

VB22B 

VB16 

VB157 

VB4B 

VB51 



VA7 

VA130 

VA259 

VB17B 

VB12B 

VB14 0 

VB33 

VB45B 

VB8B 

VB115 

VB14B 

VB22B 

VB16 

VB157 

VB4B 

VB51 



D IVMTQAAPS I PVT PGE S VS I SC 
DIVMTQAAPSVPVT PGESVS ISC 
D I VMTQAAPSVPVTPGE S VS I SC 
DIVMTQAAPS VPVT PGESVSISC 
D IVMTQAAPS VPVT PGESVSISC 
D IVMTQAAPS VPVT PGE S VS I SC 
DIVMTQAAPSVPVTPGESVSISC 
DIVMTQAAPS VPVT PGE S VS I S C 
DIVMTQAAPSVPVTPGESVSISC 
DIVMTQAAPSVPVT PGESVS ISC 
DIVMTQAAPSVPVTPGESVSISC 
D IVMTQAAPS I PVT PGE S VS I SC 
DIVMTQAAPSVPVT PGESVS ISC 
DIVMTQAAPSVSVTPGESVSISC 
D IVMTQAAPS VPVT PGESVSISC 
DIVMTQAAPSLPVTPGESVSISC 



CDR1 

RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLYSNGNIYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLHSNGNTYLY 
RS SKSLLH SNGNTYLY 
RS SKSLLH SNGNTYLY 
RSSKSLLHSNGNTYLY 
RSSKSLLYSNGNTYLY 
RSSKSLLYSNGNIYLY 
RS S KSLLHNNGNTYLY 
RSSKSLLHSNGNTYLY 



CDR2 



WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFMQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQ SPQLLIY 
WFLQRPGQSPQLLIY 
WFLQRPGQSPQLLIY 



RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 
RMSNLAS 



CDR3 



GVPDRF SGSG SGTAFTLR I SRVEAEDVG I YYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GAPDRFSGSGSGTAFTLRISRVETEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGAAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGAAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLTISSVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLKI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 
GVPDRFSGSGSGTAFTLRI SRVEAEDVGVYYC 



MQHLEYPFT 
MQHLEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHVEYPYT 
MQHLEYPYT 
MQHLEYPYT 
MQHIEYPFT 
MQHLEYPYT 
MQHLEYPYT 
MQHIEYPFT 
MQHLEYPYT 



FGTGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 
FGSGTKLEIK 



ffifE# 2005-3004069 





tBiE# 2005-3004069 



#M 2004-071763 



[19] 




0.001 0.010 0.100 1.000 10.000 100.000 
: Mfx. [nM] 




0.000 0.001 0.010 0.100 1.000 10.000 100.000 

tmfx. [nM] 



ffi|E# 2005-300 4 069 



2004-071763 



[Hill 




0.000 0.001 0.010 0.100 1.000 10.000 100.000 

/JUS [nM] 

[11 2] 



-^-hTPO 

-±-VB08Bdb 

-*-VB45Bdb 

-*-VB033db 

-«-VB140db 

-*-VB157db 

-«-TA136db 



°-1 1-0 10.0 100.0 1000.0 

Conc.(ng/ml) 



ffiliE# 2005-3004069 




#M 2004-071763 



^ — v? 




0.1 



1 4] 



1 

0.9 
0.8 
0.7 
g0-6 
20.5 
O0.4 
0.3 
0.2 
0.1 
0 



hTPO 
VB08Bdb 
VB45Bdb 
-*-VB033db 
-«-VB140db 
-«-VB157db 
-«-TA136db 



1.0 10.0 

Conc.(ng/ml) 



100.0 



1000.0 




■TA136 sc(Fv)2 



TA136 db 



-*-TPO 



o 
o 
o 
o 



o 
o 
o 



o 
o 



o 
o 



o 
o 
o 



o 
o 
o 

o 



o 
o 
o 
o 
o 



Conc.(nM) 



tfJIE# 2005-3004069 



#® 2004-071763 



1 5] 



o 

in 

Q 

O 



1.6 
1.4 
1.2 
1 

0.8 
0.6 
0.4 
0.2 
0 









f / 


/ / / 


-♦-TA136 sc(Fv)2 
-♦-TA136 db 
-*-TPO 








1 1 i _j | 





o 
o 
o 
o 



o 
o 

o 



o 

d 



o 
o 



o 
o 

o 



o 
o 
o 



o 
o 
o 

o 
o 



[IH 1 6] 
0.8 



Conc.(nM) 



o 
in 

Q 

O 



0.7 
0.6 
0.5 
0.4 
0.3 
0.2 
0.1 
0 



o 
o 
o 
o 















-•-TA136 sc(Fv)2 
-♦-TA136 db 
-5K-TPO 






I 1 ' 1 1 i 





o 
o 
o 



o 
o 



o 
o 



o 
o 
o 



o 
o 
o 

o 



o 
o 
o 

o 
o 



Conc.(nM) 



tBH#2 005-3004069 



o 

LO 

Q 
O 



[H 1 7] 
2 



1.8 
1.6 
1.4 
1.2 
1 

0.8 
0.6 
0.4 
0.2 
0 



o 
o 
o 
o 



#M 2004-071763 



^ — ~y 



10/ 









/ ae * » 






1 // 


-•-TA136 sc(Fv)2 
-♦-TA136 db 

-*-TP0 










—J L . , , 





o 
o 
o 



o 
o 



o 
o 



o 
o 
o 



o 
o 
o 

o 



o 
o 
o 
o 
o 



Conc.(nM) 



ffifE#2 005-3004069 



2004-071763 



^-v: 11/ 



[Hi 8] 

CO 




thliE# 2005-3004069 



[il 9] 



#H 2004-071763 



^-v: 12/E 




£BIE# 2005-3004069 



mm 



12 0 0 4 -0 7 1 7 6 3 . 1/£ 



ML fee ^ !tLto SfcUfifcMfol sc(Fv)2£h hftt 4cliK 

T, Diabody* * ^c(Fv)2(i^ T T ^ ^^^^ ^^^M L 
[1KB] 4L otl. 



tBiE#2 005-3004069 



12004-071763 

tftftflfiOM- #12 0 0 4 -0 7 1 7 6 3 

50400417156 



f«0 



HAfi^_h0 0 0 9 7 

TO 1 6 ^ 3 £ 1 7 0 



[R%x&%m 

i&mxi±mm] 
[^xte&m 



000003311 

M&mm&m 5T§5t if 

A 

100102978 

^ifti^m^ffll 1-1-1 mm*? < tef tf ;v 6 
100108774 

%tmwc±miftfflws l-i-i rmk^ < 6 



tB§E#2 005-3004069 



ft m 
ft * 



#M 2004-071763 
* I A i I i S 



[000003311] 
1990^ 9 E 5B 
1€j§?«IZM 5Ti5f If 



ttJSE#-f- £H|E#2 0 0 5 - 3 0 0 4 0 6 9
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Full text of "USPTO Patents Application 10551504"

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